髓鞘少突胶质细胞糖蛋白35~55肽段诱导C57BL/6小鼠实验性自身免疫性脑脊髓炎疾病表型

Induced experimental autoimmune encephalomyelitis shows the different phenotype of disease in C57BL/6 mice with myelin oligodendrocyte glycoprotein35-55

目的利用6~8周龄雌性C57BL/6小鼠建立实验性自身免疫性脑脊髓炎(EAE)模型,并对其疾病表型进行探讨,以期建立更接近多发性硬化(MS)的动物模型。方法通过皮内注射髓鞘少突胶质细胞糖蛋白(MOG)35~55肽段免疫50只雌性C57BL/6小鼠,建立EAE模型。每日按15分评分评估其疾病进展情况,观察90d,进一步通过病理学观察小鼠脑组织病变情况。结果EAE模型小鼠疾病表现为慢性病程(3只)、复发缓解病程(7只)和单相病程(11只)。部分小鼠(28只)虽然神经功能评分为0,但它们的毛色、精神和活动都发生了变化,在其脑组织中也观察到了炎性脱髓鞘病变。结论用C57BL/6雌性小鼠建立的EAE模型表现出与MS相似的疾病表型,可作为研究MS的良好动物模型。

Objective To establish an experimental autoimmune encephalomyelitis(EAE)model in female C57 BL/6 mice aged 6-8 weeks and investigate its disease phenotype so as to build a better animal model of multiple sclerosis(MS).Methods The EAE model was established in 50 female C57 BL/6 mice through intradermal injection of myelin oligodendrocyte glycoprotein 35-55 peptide.We assessed the disease progression daily according to a 15-point score for 90 days,and further observed the brain lesions in terms of pathology.Results There were three courses of disease in the EAE model:chronic course(3 mice),relapse and remission course(7 mice)and monophasic course(11 mice).Interestingly,we identified some mice(28 mice)had changes in coat color,mental and motor activity,as well as inflammatory demyelinating lesions in the brain tissue although their neurological functions were scored as 0 point.Conclusion The EAE model in C57 BL/6 female mice shows disease phenotype similar to multiple sclerosis and can be used as a good animal model.