生物粉末活性炭对二级出水中抗性基因转移扩散的影响

Effect of BPAC on Transfer and Diffusion of Antibiotic Resistant Genes in Secondary Effluent

针对生物粉末活性炭(BPAC)是否能促进污水厂二级出水中抗生素抗性基因(ARGs)转移扩散的相关问题,文章采用模拟水环境的方式,考察在投加BPAC的情况下,污水厂二级出水中ARGs的浓度与微生物群落结构的变化趋势;并且探究了其在不同时间、pH值和温度条件下,对水中ARGs浓度和微生物多样性及丰度的影响。结果发现,二级出水在投加BPAC后,水中ARGs浓度会降低,但同时微生物也会促进ARGs的扩散增加;水中的微生物种群丰度和多样性随反应时间的增加而增长;低温环境(4℃)和碱性环境(pH=9)更有利于ARGs的转移扩散。研究表明,BPAC对二级出水中ARGs的转移扩散起到一定的促进作用,但其作用效果也受时间、环境等状况的限制和影响。

To solve the problem of whether biological powder activated carbon(BPAC)can promote the transfer and diffusion of antibiotic resistance gene(ARGs)in secondary effluent of wastewater treatment plant,the change trend of ARGs concentration and microbial community structure in secondary effluent of wastewater treatment plant under BPAC condition was investigated by simulating the water environment,and effects of the ARGs concentration and microbial diversity and abundance were also discussed under various time,pH value and temperature.The results showed that the concentration of ARGs in secondary effluent decreased after the addition of BPAC,but the microorganism also promoted the diffusion of ARGs.The abundance and diversity of microbial population in water increased with the increase of reaction time.Low temperature environment(4℃)and alkaline environment(pH=9)were more conducive to the transfer and diffusion of ARGs.The research showed that BPAC can promote the transfer and diffusion of ARGs in secondary effluent,but its effect was limited and affected by time,environment and other conditions.