电针督脉四穴组对脊髓损伤后大鼠运动及神经传导功能恢复的作用机制研究

Study on the Mechanism of Action of Electroacupuncturing Four Acupionts Group of Du-meridian for Provement of Motor and Nerve Conduction Function in Rats after Spinal Cord Injury

目的:分析电针对脊髓损伤后大鼠运动及神经传导功能恢复的改善作用。方法:将24只SD大鼠随机分为假手术组、模型对照组、电针组;假模型组行椎板切除术但不对其脊髓进行打击及电针治疗,模型组行正常造模但不行电针治疗干预,电针组在模型组基础上进行电针治疗;干预结束后对后肢运动功能评价,并检测神经传导功能和受损脊髓组织中BDNF、NT-3mRNA及蛋白水平。结果:术后三周及术后七周时电针组BBB评分明显高于模型组,且差异存统计学意义(P<0.05);术后三周及术后七周时电针组大鼠SEP潜伏期明显低于模型组,且差异存统计学意义(P<0.05);术后三周及术后七周时电针组大鼠SEP波幅明显高于模型组,且差异存统计学意义(P<0.05);治疗后电针组大鼠BDNF、NT-3mRNA及蛋白水平明显高于模型组,且差异存统计学意义(P<0.05)。结论:采用电针对脊髓损伤大鼠干预后可有效改善大鼠运动功能,并明显提高大鼠神经传导功能恢复。

Objective:To analyze the effect of electro-acupuncture on motor and nerve conduction function recovery after spinal cord injury in rats.Methods:Twenty-four SD rats were randomly divided into sham-operated group,model controlled group and electro-acupuncture group.The sham-model group underwent laminectomy but did not undergo striking and electroacupuncture treatment on their spinal cord.The model group received normal modeling but no electro-acupuncture treatment intervention.The electro-acupuncture group received electro-acupuncture treatment on the basis of the model group.The motor function of hind limbs was evaluated after the intervention.The levels of BDNF,NT-3 mRNA and protein in nerve conduction function and injured spinal cord tissue were detected.Results:BBB score of the EA group was significantly higher than that of the model group at 3 weeks and 7 weeks after operation,and the difference was statistically significant(P<0.05);SEP latency of the EA group was significantly lower than that of the model group at 3 weeks and 7 weeks after operation(P<0.05);SEP amplitude of the EA group was significantly higher than that of the model group at 3 weeks and 7 weeks after operation(P<0.05).The levels of BDNF,NT-3 mRNA and protein in the EA group were significantly higher than those in the model group(P<0.05).Conclusion:The motor function and nerve conduction function of rats can be effectively improved after the intervention of electro-acupuncture on spinal cord injury rats.