长苞铁杉中木脂素类化学成分研究

Lignans from the Heartwood of Nothotsuga longibracteata

从中国特有植物长苞铁杉心材中分离纯化得到1个新的苄基呋喃酮衍生物(1),2个新的木脂素(2,3),以及19个已知的木脂素类化合物,新化合物的结构通过HR-ESI-MS,1D NMR,2D NMR,CD等光谱技术分别鉴定为3-(4'-羟基-3'-甲氧基苄基)-2(5H)-呋喃酮(1),(8S,8'S)-3,3'-二甲氧基木脂-4,4',9,9'-四醇-9-乙酸酯(2),(7'R,8S,8'S)-3',5-二甲氧基-2,7'-环木脂-4,4',9'-三醇-9-酸丁酯.噻唑蓝(MTT)法测试显示新化合物对三种肿瘤细胞(A172,SHSY5Y,Hela)均未表现出明显毒性.斑马鱼肠胃蠕动活性测试显示,化合物3在8和24µmol/L浓度下对斑马鱼肠胃蠕动有明显的促进作用.

3-(4'-Hydroxy-3'-methoxybenzyl)-2(5H)-furanone(1),a rare benzyl furanone derivate,and two new lignans(2 and 3)were isolated from the heartwood of Nothotsuga longibracteata,together with nineteen known lignans compounds.The structures and absolute configurations of the undescribed compounds were elucidated on the basis of HR-ESI-MS,1D NMR,2D NMR,and CD spectroscopies.The cytotoxic effects of the isolated new lignan compounds on three human tumour cell lines(A172,SHSY5Y,and Hela)were evaluated by methyl thiazolyl tetrazolium(MTT)assay.Their showed no cytotoxic activities at the concentration of 50μmol/L.Gastrointestinal motility test of zebrafish showed that compound 3 has the function of promoting gastrointestinal motility of zebrafish to excrete Nile red at doses 8 and 24µmol/L by means of acting on the cholinergic nervous system.