牛膝治疗膝骨关节炎的网络药理学机制研究

Mechanism research on network pharmacology of Achyranthis bidentatae radix intreating knee osteoarthritis

目的探讨牛膝治疗膝骨关节炎的有效活性成分及作用机制。方法检索TCMSP中牛膝含有的化合物,筛选其活性成分,进而获取活性成分的潜在靶点;通过GeneCards数据库挖掘膝骨关节炎靶点;结合化合物及疾病靶点,构建化合物-靶点网络,进行GO富集及KEGG通路富集分析。结果牛膝含有23个活性成分,对应膝骨关节炎136个靶点;获得1592条GO生物过程相关条目与19条信号通路,涉及IL-17信号通路、TNF信号通路、凋亡信号通路、PI3K/AKT信号通路等通路。结论牛膝主要通过调控膝关节内免疫炎症反应减轻炎性损伤,并抑制软骨细胞凋亡,保护软骨。

Objective To investigate the active ingredients and mechanisms of Achyranthis bidentatae radix in treating knee osteoarthritis(KOA).Methods To detect theingredients in Achyranthis bidentatae radix from TCMSP and screen its active components so as to abtain the potential targets of those active components.By searching from the TCMSP,to explore the targets of KOA.Then,by integrating the chemical compound and disease targets to construct its network and to analize the gene ontology(GO)enrichment and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment.Result There are 23 active compounds in Achyranthis Bidentatae radix,corresponding to 136 targets of KOA.By GO enrichment analysis,there are 1592 GO terms related to biological process.By KEGG pathway enrichment analysis,there are 19 KEGG pathways,including IL-17 signaling pathway,TNF signaling pathway,Apoptosis,and PI3K/AKT signaling pathway.Conclusions The Achyranthis bidentatae radix could treat KOA by reducing inflammatory damage by regulating immune inflammatory response in the knee joint,and inhibiting chondrocyte apoptosis to protect cartilage.