小麦转录因子基因TaMYB70的分离和表达分析

Isolation and Expression Analysis of Transcription Factor Gene TaMYB70 in Wheat

为了探究小麦MYB转录因子基因TaMYB70的功能,采用同源克隆方法分离TaMYB70(MK024291)基因cDNA序列,运用生物信息学手段分析该基因序列特征,采用实时荧光定量反转录PCR(qRT-PCR)检测其在不同逆境胁迫下的表达模式。结果表明,分离得到的TaMYB70部分序列长度为1272bp,包含一个长度为1008bp的开放阅读框,编码335个氨基酸残基。TaMYB70蛋白含有2个螺旋-转角-螺旋结构的Mybtype HTH DNA结合结构域。TaMYB70氨基酸序列与粗山羊草、二穗短柄草等植物MYB44同源性较高,与拟南芥R2R3-MYB转录因子第22亚族成员属于同一分支。qRT-PCR分析表明,TaMYB70在脱落酸胁迫下表达量升高,在PEG和NaCl胁迫下表达量下降,该转录因子可能参与小麦逆境胁迫应答。

To excavate the function of MYB transcription factor TaMYB70 in wheat,TaMYB70 gene was isolated using homology cloning strategy.The expression patterns of TaMYB70 under different stresses were examined by quantitative real-time PCR(qRT-PCR).The results showed that the TaMYB70 partial cDNA was 1272 bp and contained an open reading frame of 1108 bp,encoding 335 amino acids.TaMYB70 protein contained two Myb-type HTH DNA binding domains which were helix-turn-helix motifs.The homologous sequence alignment indicated that the TaMYB70 had higher homology with MYB44 from other plants,such as Aegilops tauschii and Brachypodium distachyum(L.).TaMYB70 belongs to the same branch as the 22nd members of the arabidopsis R2R3-MYB transcription factors.The expression of TaMYB70 was upregulated under ABA treatment,and downregulated under PEG and NaCl stresses,and which may be involved in stress response of wheat.