花魔芋花芽和叶芽的转录组分析

Transcriptome Analysis of Flower Buds and Leaf Buds of Konjac(Amorphophallus konjac)

魔芋是天南星科多年生草本植物,特有的魔芋葡甘露聚糖具有广泛的应用。然而魔芋生活周期较长,影响魔芋有性杂交育种工作的开展。针对魔芋花芽分化的机制尚不明确这一问题,本研究以花魔芋为试验材料,用二代测序技术对花芽和叶芽进行转录组测序和基因功能分析,共获得61.03 Gb的数据,组装后得到184 366条Transcript和95 707条Unigene序列,其中29 685条Unigene注释成功,占全部Unigene的31.00%。差异基因表达分析发现,与花芽相比,叶芽中有781个基因的表达上调,1 822个基因的表达下调。KEGG信号通路分析发现,35个基因参与植物激素信号转导通路,暗示激素可能在花芽和叶芽的分化过程中发挥重要作用。本研究为深入开展魔芋花芽分化的分子机制研究提供了一定的理论基础。

Konjac,a perennial herb of Araceae,produces konjac glucomannan which has wide applications.How-ever,the long-life cycle of konjac affects the development of sexual crossbreeding.To solve the problem that the mechanism of flower bud differentiation is not clear,transcriptome of flower buds and leaf buds of Amorphop-hallus konjac were sequenced using high throughput sequencing technology in this study.A total of 61.03 Gb of data were obtained,de novo assembly generated 184366 Transcripts and 95707 Unigenes,of which 29685 Unigenes annotations were obtained,accounting for 31.00%.Differential gene expression analysis showed that 781 genes were up-regulated and 1822 genes were down-regulated in leaf buds compared with flower buds.KEGG signaling pathway analysis revealed that 35 genes were involved in plant hormone signaling pathway,suggesting that hormones may play an important role in flower bud and leaf bud differentiation.This study will provide a theoretical basis for further study on the molecular mechanism of flower bud differentiation of konjac.