三种黑麦种质的染色体FISH核型分析

FISH Karyotype Analysis in Three Kinds of Rye Germplasm

为了解黑麦染色体的FISH核型特点,本研究使用Oligo-pSc119.2-2、Oligo-pSc200和Oligo-pSc250探针对3种黑麦(MAD黑麦, WR7黑麦和C718黑麦)染色体进行了双色FISH分析,结果发现3份黑麦的7对染色体上,Oligo-pSc119.2-2红色信号强且丰富,广泛分布于染色体端部及中部等。Oligo-pSc200和Oligo-pSc250绿色信号强,主要位于染色体端部及近端部。3份黑麦每条染色体的Oligo-pSc119.2-2信号分布较为相似,但是Oligo-pSc200和Oligo-pSc250信号的差别较大。MAD黑麦和WR7黑麦染色体上的Oligo-pSc200和Oligo-pSc250信号比C718黑麦多且强。MAD黑麦和WR7黑麦的FISH核型较为相似,它们与C718黑麦的差别较大。通过本试验了解到3份黑麦的FISH核型之间存在遗传多样性,建立了3份黑麦的FISH核型,这将有助于该种质在麦类作物遗传育种上的利用和深入研究。

To understand the characteristics of FISH karyotype of rye chromosomes,bicolor FISH analysis was performed on the chromosomes of three rye varieties(MAD rye,WR7 rye and C718 rye)using the probes Oligo-psc119.2-2,Oligo-psc200 and Oligo-psc250.It was found that the red signals of Oligo-pSc119.2-2 probe were strong and abundant on 7 pairs of chromosomes in three rye varieties,which were widely distributed at chromo-some ends,interstitial sections and so on.The Oligo-pSc200 and Oligo-pSc250 probes had strong green signals,and were mainly located at the ends and proximal ends of the chromosomes.Oligo-pSc119.2-2 signal distributions on each chromosome of three ryes were similar,but those of Oligo-pSc200 and Oligo-pSc250 showed quite dif-ferent.The Oligo-pSc200 and Oligo-pSc250 signals of MAD rye and WR7 rye chromosomes were more and stronger than those of C718 rye.The FISH karyotype patterns of MAD rye and WR7 rye were similar,but they had more differences from C718 rye.Through this experiment,the genetic diversity of FISH karyotypes in three ryes was known,and the FISH karyotypes in three ryes were established,which would be helpful for the utilization and further study in genetics and breeding of Triticeae crops.