SH3结构域谷氨酸富集样蛋白3在非小细胞肺癌中的表达及意义

Expression of SH3BGRL3 in Non-small Cell Lung Cancer and Its Significance

[目的]评价SH3结构域谷氨酸富集样蛋白3(SH3BGRL3)在非小细胞肺癌中的表达及其与临床病理特征的相关性。[方法]选取2014年10月至2017年12月确诊并接受手术治疗的46例非小细胞肺癌患者的癌组织标本以及距离癌组织5cm以上的癌旁组织,免疫组化染色检测SH3BGRL3相对表达量,荧光定量RT-PCR检测SH3BGRL3 m RNA表达。[结果]癌组织中SH3BGRL3相对表达量、SH3BGRL3 mRNA表达高于癌旁组织(P<0.05)。肿瘤体积≥5cm3、侵及浆膜、病理分期Ⅲ~Ⅳ期、有淋巴结转移患者SH3BGRL3、SH3BGRL3 m RNA相对表达量高于肿瘤体积<5cm3、未及浆膜、病理分期Ⅰ~Ⅱ期、无淋巴结转移患者(P<0.05)。病理分期、SH3BGRL3、SH3BGRL3 mRNA表达是影响非小细胞肺癌患者预后的主要危险因素。[结论]SH3BGRL3在非小细胞肺癌中呈现异常高表达,且与肿瘤体积、浸润程度、病理分期、淋巴结转移等临床病理参数具有一定相关性。SH3BGRL3、SH3BGRL3 m RNA表达是影响非小细胞肺癌患者预后的危险因素。

[Objective]To investigate the expression of SH3 domain-binding glutamic acid rich like protein 3(SH3 BGRL3)in non-small cell lung cancer(NSCLC)and its significance.[Methods]From October 2014 to December 2017,46 patients with non-small cell lung cancer(NSCLC)were enrolled in the study.The expression of SH3 BGRL3 protein and m RNA was detected by immunohistochemical staining and fluorescence quantitative RT-PCR in cancer tissue specimens and pericancerous tissue specimens,respectively.[Results]The expression of SH3 BGRL3 protein and the SH3 BGRL3 m RNA in cancer tissues were significantly higher than that in adjacent tissues(P<0.05).The expression of SH3 BGRL3 protein and m RNA in patients with lymph node metastasis,serosa invasion,tumor volume>5 cm3 and pathological stageⅢ~Ⅳwas higher than that in those without lymph node metastasis and serosa invasion,and with tumor volume<5 cm3 and pathological stageⅠ~Ⅱ(all P<0.05).Multivariate regression analysis showed that pathological stage,SH3 BGRL3 m RNA and protein expression were independent risk factors affecting the prognosis of patients with non-small cell lung cancer.[Conclusion]SH3 BGRL3 is highly expressed in non-small cell lung cancer,and it is correlated with tumor volume,invasion degree,pathological stage and lymph node metastasis.The expression of SH3 BGRL3 and SH3 BGRL3 is the risk factor affecting the prognosis of non-small cell lung cancer.