摘要
大豆是食用植物蛋白质和油脂的主要来源,提高大豆蛋白质和油分含量是主要的育种目标,与传统育种相比,利用分子标记定位QTL辅助育种,在实用价值和理论意义上都对大豆育种具有十分重要的价值。利用蛋白质与油分含量差异较大的大豆亲本东农L13和合农60、黑河36,分别构建了以东农L13为共同亲本的2个重组自交系群体RIL3613(东农L13×黑河36)和RIL6013(东农L13×合农60),分别包含134,156个株系;利用3个生态环境下数据对大豆蛋白含量和油分含量进行了表型数据分析,分别利用150,137个SSR标记构建遗传图谱,采用完备区间作图法(ICIM),对3个环境下的油分和蛋白质含量进行了QTL定位。通过对表型数据的分析,2个RIL群体的蛋白质与油分含量在基因型间或不同环境条件下的差异均达极显著水平,且基因型与环境间存在极显著的互作效应。2个群体中,共检测到8个蛋白质含量QTL,分布于7个连锁群上;共检测出5个控制油分含量的QTL,分布于5个连锁群上,有1个油分含量的QTL在2个种植环境下重复检测到。在定位的QTL中,7个蛋白质含量相关的QTL和3个油分含量相关的QTL与前人研究一致,另外3个QTL(qPro-G-1、qOil-C1-1、qOil-H-1)是本研究新发现的,是本研究遗传背景特有的QTL。研究结果对大豆品质性状的分子设计育种具有重要意义。
Soybean is the main source of protein and oil in edible plants,improvement of protein and oil content are the major objective of soybean breeding programs.Mapping QTL by molecular markers has important theoretical significance and practical value for soybean molecular breeding.Here,two associated soybean recombinant inbred line(RIL)populations,Dongnong L13×Henong 60(RIL6013)and Dongnong L13×Heihe 36(RIL3613),comprising 134,156 RILs,respectively,were grown in three environments and their protein and oil contents were measured.The genetic map was constructed with 150,137 SSR markers respectively,and the complete interval mapping(ICIM)was used to map the oil and protein content.Through the analysis of phenotypic data,the phenotypic variation coefficients of protein and oil content in the two RIL populations were large,and there were significant differences in protein content and oil content in different environments.8 QTLs for protein content were found in both RIL3613 and RIL6013 on 7 linkages,and 5 for oil content were found on 5 linkages.1 QTL for oil content was repeatedly detected in 2 planting environments.In the located QTLs,7 protein content and 3 oil content QTLs were consistent with previous studies,and the other three(qPro-G-1,qOil-C1-1,qOil-H-1)were newly discovered in this study and were specific to the genetic background of this study.These results are of great significance for molecular design breeding of soybean quality traits.
作者
田雨
王艳殊
张佳楠
许世超
董全中
李文滨
李文霞
宁海龙
TIAN Yu;WANG Yanshu;ZHANG Jianan;XU Shichao;DONG Quanzhong;LI Wenbin;LI Wenxia;NING Hailong(College of Agriculture,Northeast Agricultural University,Key Laboratory of Soybean Biology,Ministry of Education,Key Laboratory of Soybean Biology and Breeding (Genetics),Ministry of Agriculture,Harbin 150030,China;Keshan Branch of Heilongjiang Academy of Agricultural Sciences, Keshan 161606,China)
出处
《华北农学报》
CSCD
北大核心
2020年第4期106-112,共7页
Acta Agriculturae Boreali-Sinica
基金
黑龙江省自然科学基金留学归国项目(LC2016010)。