摘要
目的探讨乙醇注入法制备虾青素脂质体的处方与工艺。方法采用乙醇注入法制备虾青素脂质体,以脂质体包封率为考察指标,运用单因素实验与正交实验筛选虾青素脂质体的处方与工艺,并对按优选处方与工艺制备的虾青素脂质体进行表征。结果虾青素脂质体最佳处方与工艺:脂药比为40:1、磷脂与胆固醇的质量比为20:1、水合介质pH值为7.0、温度为60℃,平均包封率为(35.28±0.93)%,平均粒径为143.2 nm。结论乙醇注入法制备虾青素脂质体工艺简单,适合于工业化制备虾青素脂质体。
Objective To investigate the prescription and process of astaxanthin liposome by ethanol injection method.Methods Astaxanthin liposome was prepared by ethanol injection.Encapsulation efficiency was taken as index to screen the prescription and process of astaxanthin liposome by single factor test and orthogonal test.The characterization of astaxanthin liposome was measured.Results The optimal prescription and process were as followed:the ratio of lipids to astaxanthin was 40:1;the ratio of phospholipid to cholesterol was 20:1;pH of hydration medium were 7.0.The temperature was 60℃.Average encapsulation efficiency was(35.28±0.93)%and mean particle size was about 143.2 nm.Conclusion The process of astaxanthin liposome prepared by ethanol injection method is simple,and suitable for industrial manufacture.
作者
杨安平
顾斯颖
梁一俊
杜卓
YANG Anping;GU Siying;LIANG Yijun;DU Zhuo(School of Pharmaceutical Engineering,Foshan University,Foshan 528000,China)
出处
《医药导报》
CAS
北大核心
2020年第9期1276-1280,共5页
Herald of Medicine
基金
国家自然科学基金资助项目(81901881)
广东省社会发展领域科技计划项目(0903129)。
关键词
虾青素
脂质体
乙醇注入法
包封率
Astaxanthin
Liposome
Ethanol injection method
Encapsulation efficiency
