摘要
目的为建立制备大量高纯度黄芩新素的方法。方法比较了超声法和回流提取法提取黄芩中黄芩新素,及不同萃取溶剂对黄芩中黄芩新素得率的影响,通过HPLC色谱法测定黄芩新素的含量,并用LC-8A高压制备色谱仪制备黄芩新素。结果采用回流提取法及使用乙醚作为萃取溶剂时,黄芩新素得率最高,制备的黄芩新素纯度可达到97.96%。结论本实验建立的方法操作简便,重现性好,制备的黄芩新素纯度符合对照品纯度的要求。
Objective:To establish a method for preparing a large amount of high-purity neobaicalein.Methods:The extraction of neobaicalein from Scutellaria baicalensis by ultrasonic method and reflux extraction method were compared,and the effect of different extraction solvents on the yield of neobaicalein from S.baicalensis was investigated.The content of neobaicalein was determined by HPLC,and neobaicalein was prepared by LC-8 A high-pressure preparative chromatograph.Results:When reflux extraction was adopted and ether were used as extraction solvent,the yield of neobaicalein was the highest,and the purity of neobaicalein was 97.96%.Conclusion:The method established in this experiment is easy to operate and has good reproducibility.And the purity of the prepared neobaicalein mets the requirements of the reference substance.
作者
黄雪雪
陈莉
林珠灿
余丽双
HUANG Xue-xue;CHEN Li;LIN Zhu-can;YU Li-shuang(College of pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China)
出处
《井冈山大学学报（自然科学版）》
2020年第4期91-94,共4页
Journal of Jinggangshan University (Natural Science)
基金
国家自然科学基金项目(81903855)
福建省科技厅计划项目(2017Y0050,2018J01871)。
关键词
黄芩
黄芩新素
HPLC
scutellaria baicalensis
neobaicalein
HPLC
