益气化瘀豁痰方对动脉粥样硬化大鼠的抗炎抗氧化应激作用研究

Yiqi Huayu Huotan Prescription Has Anti-Inflammatory and Antioxidative Stress Effect on Rats with Atherosclerosis

目的:观察益气化瘀豁痰方对动脉粥样硬化(AS)大鼠的抗炎抗氧化应激作用。方法:将24只雄性Wistar大鼠随机分为对照组、模型组和给药组,每组8只。模型组和给药组大鼠喂养高脂饲料,并按2 mL/kg的剂量腹腔注射60 IU/kg的维生素D3,制备模型12周。给药组大鼠在模型制备过程中每天灌胃100 mg/kg的益气化瘀豁痰方,对照组和模型组灌胃相同剂量的生理盐水。12周后采集大鼠血清,HE染色观察大鼠主动脉形态结构;采用ELISA法检测各组大鼠血清中高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、甘油三酯(TG)、C-反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)和活性氧(ROS)的含量;采用蛋白免疫印迹法检测大鼠主动脉核因子κB(NF-κB)的表达。结果:与对照组比较,模型组大鼠主动脉病变部位出现内膜增厚破坏,内皮细胞排列不完整;LDL-C、TC、TG、CRP、TNF-α、IL-6、NF-κB、MDA和ROS水平明显升高(P<0.05);HDL-C、SOD、CAT水平显著降低(P<0.05)。与模型组比较,给药组血管内膜光滑平整;LDL-C、TC、TG、CRP、TNF-α、IL-6、NF-κB、MDA和ROS水平显著下降(P<0.05);HDL-C、SOD、CAT水平明显上升(P<0.05)。结论:益气化瘀豁痰方可通过抑制炎症反应和改善氧化应激来预防大鼠AS。

Objective:To observe the anti-inflammatory and antioxidative stress effect of Yiqi Huayu Huotan prescription on rats with atherosclerosis(AS). Methods:A total of 24 male Wistar rats were randomly divided into the control group,the model group and the administration group,8 rats in each group. In the model group and the administration group,the rat models were established by high fat diet and intraperitoneal injection of 60 IU/kg vitamin D3 at a dose of 2 mL/kg,for 12 weeks. The administration group was given 100 mg/kg Yiqi Huayu Huotan prescription by gavage per day during the model establishment,and the control group and the model group were given saline of the same volume by gavage. After 12 weeks,the serum of rats was collected for HE staining, and then the morphometric structure of aorta was observed. The ELISA method was applied to detect the contents of and high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C),total cholesterol(TC),triglyceride(TG),C-reactive protein(CRP),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6), superoxide dismutase(SOD), catalase(CAT), malondialdehyde(MDA) and reactive oxygen species(ROS) in serum. The protein immunoblotting method was used to detect the expression of nuclear factor κB(NF-κB) in aorta.Results:Compared with those in the control group,in the observation group,there were intimal thickening and damage as well as incomplete arrangement of endothelial cells at the lesion site of aorta;the levels of LDL-C,TC,TG,CRP,TNF-α,IL-6,NF-κB,MDA and ROS in the observation group were significantly increased(P<0.05),and the levels of HDL-C,SOD and CAT were significantly decreased(P<0.05). Compared with those in the model group,in the administration group,the intima was smooth;the levels of LDL-C,TC,TG,CRP,TNF-α,IL-6,NF-κB,MDA and ROS were significantly decreased(P<0.05),and the levels of HDL-C,SOD and CAT were significantly increased(P<0.05).Conclusion:Yiqi Huayu Huotan prescription can prevent AS in rats via the inhibition of inflammation and the improvement of oxidative stress.