大孔树脂NKA-9负载RML催化高酸价油脂脱酸及制备甘油二酯

Enzymatic Deacidification of High-Acid Oil for Diacylglycerol Production Using NKA-9 Macroporous Resin-Supported Rhizomucor miehei

本文采用了大孔树脂NKA-9作为载体负载脂肪酶RML,然后用于催化油酸含量为25%的大豆油脂以酶促脱酸并同时制备富含甘油二酯(DAG)的油脂产品。研究采用了扫描电镜(SEM)和X-射线光电能谱仪(XPS)对NKA-9负载酶前后进行了结构表征,结果表明RML已成功负载在NKA-9上。所得固定化酶RML@NKA-9催化油酸含量为25%的大豆油脱酸及DAG制备的优化条件为:反应温度50℃,甘油用量为油脂质量的8%,RML@NKA-9用量为油脂质量的3%,在该条件下反应8 h后达到平衡,此时DAG含量58.95%,游离脂肪酸(FFA)含量降低至1.50%。此外研究了RML@NKA-9的重复利用性,结果表明,RML@NKA-9重复使用4次后其酶活几乎没有损失,重复使用6次后其酶活为初始酶活的49.10%。本研究制备的RML@NKA-9能有效脱除高酸价油脂中的FFA,并获得了可观含量的DAG,具有一定的重复利用性,因此,RML@NKA-9具有较大的应用前景。

Macroporous resin NKA-9 was used as the support for the immobilization of lipase from the fungus Rhizomucor miehei(RML).The resin-supported RML(RML@NKA-9)was then used in the enzymatic deacidification of high-acid soybean oil with an oleic acid content of 25%for diacylglycerol(DAG)production.Structural characterization of the resin before and after RML loading using SEM and XPS revealed that RML had been successfully immobilized onto NKA-9.The optimized reaction conditions for enzymatic deacidification and DAG production were as follows:reaction temperature,50°C;amount of glycerol,8 wt%of the high-acid oil;and amount of RML@NKA-9,3 wt%of the high-acid oil.Under these conditions,equilibrium was reached after 8 h of reaction,DAG content of 58.95%was achieved,and free fatty acid(FFA)content was reduced to 1.50%.Subsequently,the reusability of RML@NKA-9 was evaluated.Results indicated almost no activity loss after four cycles of use,and the retention of 49.10%of initial activity after six cycles of use.The RML@NKA-9 prepared in this study enabled the effective removal of FFAs from high-acid oil,achieved the production of considerable DAG content,and demonstrated a certain degree of reusability.Therefore,RML@NKA-9 possesses promising prospects for applications in oil processing.