磷酸三(2,3-二氯丙基)酯(TDCIPP)和纳米二氧化钛(nano-TiO2)联合暴露对斑马鱼胚胎的氧化应激影响

Effects of tri(2,3-dichloropropyl)phosphate(TDCIPP)and nano-TiO2 on oxidative stress in zebrafish embryos

主要研究了磷酸三(2,3-二氯丙基)酯(TDCIPP)和纳米二氧化钛(nano-TiO2)对斑马鱼胚胎的氧化应激影响.实验设置了5μg/L、20μg/L、80μg/L、180μg/L的TDCIPP与1 mg/L nano-TiO2的单独暴露组,以及TDCIPP与nano-TiO2的复合暴露组.结果表明:斑马鱼胚胎暴露于TDCIPP与nano-TiO2都会导致活性氧(ROS)水平升高、抗氧化酶水平改变以及相关基因转录;与对照组相比,nano-TiO2会增加ROS的含量,不过影响较小,TDCIPP单独暴露会诱导ROS含量增加;TDCIPP与nano-TiO2复合暴露时,ROS含量增加的幅度变小;与ROS的变化趋势相似,复合暴露组超氧化歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)的活性以及丙二醛(MDA)的水平也较TDCIPP单一暴露有所降低.本文通过在基因水平上检测了TDCIPP与nano-TiO2对Mn-Sod、Cu/Zn-Sod、Cat这三种抗氧化酶基因表达的影响,结果显示,nano-TiO2可以削弱TDCIPP对斑马鱼的氧化伤害.

In the experiment,the oxidative stress of TDCIPP and nano-TiO2 on zebrafish embryos was studied.The separately groups of 5,20,80,180μg/L TDCIPP and 1 mg/L nano-TiO2,and combination group of TDCIPP and nano-TiO2 were set.The results are as follows:the exposure of zebrafish embryos to TDCIPP and nano-TiO2 can lead to the increase of ROS,the change of antioxidant enzymes and the transcription of related genes.Compared with the control group,nano-TiO2 can increase the content of ROS,but the effect is small.TDCIPP alone can induce the increase of ROS.The increase of ROS content decreased when TDCIPP and nano-TiO2 composite exposure.Similar to the trend of ROS,the activities of SOD,GPx and MDA in the compound exposure group were lower than those in the TDCIPP single exposure group.In this paper,the effects of TDCIPP and nano-TiO2 on the expression of Mn-Sod,Cu/Zn-Sod and Cat were detected at the gene level.The results show that nano-TiO2 could weaken the oxidative damage of TDCIPP to zebrafish.