LncRNA RP11-316M1.12在甲状腺乳头状癌中的表达及对细胞侵袭、迁移的影响

Expression of LncRNA RP11-316M1.12 in Papillary Thyroid Carcinoma and Its Effects on Cell Invasion and Migration

目的:探讨长链非编码RNA(LncRNA)RP11-316M1.12在甲状腺乳头状癌(PTC)中的表达及对细胞侵袭、迁移的影响。方法:采用荧光定量PCR(qRT-PCR)法检测42例PTC组织及其相应癌旁组织中LncRNA RP11-316M1.12表达水平。体外培养TPC-1细胞,将TPC-1细胞分为LncRNA RP11-316M1.12-siRNA组(敲低组)、阴性对照组(NC组)、空白对照组(NG组),qRT-PCR法检测各组TPC-1细胞中LncRNA RP11-316M1.12表达水平,CCK-8法检测各组TPC-1细胞增殖能力,Transwell实验检测各组TPC-1细胞迁移、侵袭能力,Western blot法检测各组TPC-1细胞中上皮-间充质转化(EMT)相关蛋白表达水平。结果:与癌旁组织相比,PTC癌组织中LncRNA RP11-316M1.12相对表达量明显升高(P<0.05)。与NC组、NG组比较,转染24、48、72 h敲低组TPC-1细胞增殖能力受到抑制(P<0.05)。与NC组、NG组比较,敲低组迁移细胞数、侵袭细胞数、间质细胞标志物波形蛋白(vimentin)、N-钙粘附蛋白(N-cadherin)蛋白相对表达量均显著降低(P<0.05),上皮细胞标志物E-钙粘附蛋白(E-cadherin)相对表达量显著升高(P<0.05)。结论:LncRNA RP11-316M1.12在PTC癌组织中呈高表达,沉默LncRNA RP11-316M1.12可抑制TPC-1细胞增殖、迁移、侵袭能力,其机制可能与PTC肿瘤细胞EMT过程有关。

Objective:To investigate the expression of long non-coding RNA(LncRNA)RP11-316M1.12 in papillary thyroid carcinoma(PTC)and its effects on cell invasion and migration.Methods:The expression levels of LncRNA RP11-316M1.12 in PTC tissues and its corresponding paracancerous tissues of 42 cases were detected by fluorescence quantitative PCR(qRT-PCR).TPC-1 cells were cultured in vitro and divided into LncRNA RP11-316M1.12-siRNA group(Knockdown group),negative control group(NC group),blank control group(NG group),the expression of LncRNA RP11-316M1.12 in each group of TPC-1 cells was detected by qRT-PCR,CCK-8 method was used to detect the proliferation of each group of TPC-1 cells,the cell migration and invasion of each group of TPC-1 cells was detected by Transwell test,Western blot method was used to detect the expression levels of epithelial-mesenchymal transition(EMT)related proteins in each group of TPC-1 cells.Results:Compared with the paracancerous tissues,the relative expression of LncRNA RP11-316M1.12 was significantly higher in PTC tissues(P<0.05).Compared with NC group and NG group,the proliferation of TPC-1 cells was inhibited in knockdown group after transfection for 24,48,72 h(P<0.05).Compared with NC group and NG group,the number of migrating cells,invasive cells,the relative expressions of stromal cell markers vimentin(vimentin)and N-epithelial cadherin(N-cadherin)decreased significantly(P<0.05),the relative expression of epithelial cell marker E-epithelial cadherin(E-cadherin)increased significantly(P<0.05).Conclusions:LncRNA RP11-316M1.12 is highly expressed in PTC tissues,silent LncRNA RP11-316M1.12 can inhibit the proliferation,migration and invasion of TPC-1 cells and its mechanism may be related to the EMT processes of PTC tumor cells.