半导体激光联合全蝎软膏对大鼠糖尿病性皮肤溃疡修复的研究

Repair of Diabetic Skin Ulcer in Rats by Semiconductor Laser Combined with Scorpion Ointment

目的:探讨半导体激光联合全蝎软膏对大鼠糖尿病皮肤溃疡(DSU)的修复作用。方法:24只SPF级Wistar大鼠随机平均分为对照组、模型组、全蝎软膏组和联合组,以腹腔注射链脲佐菌素-皮肤缺损法构建大鼠DSU模型;建模后分别用激光和/或全蝎软膏对DSU大鼠进行治疗,观察用药后3 d、7 d、10 d和14 d的大鼠体重、血糖值及创面肉芽组织愈合情况;随后,分别对创面组织进行H.E染色、免疫组化、ELISA法及western blot检测。结果:建模后的DSU大鼠体重、血糖与对照组比较有统计学差异(P<0.05),且成模率为73.33%;各治疗组创面愈合率均高于模型组(P<0.05),肉芽组织生长旺盛,毛细血管丰富,炎症反应减轻;ELISA和western blot结果显示,与模型组相比,联合组治疗后组织内血管内皮生长因子(vEGF)显著上升(P<0.05)、肿瘤坏死因子α(TNF-α)和Smad 4蛋白水平显著降低(P<0.05),治疗效果优于全蝎软膏组。结论:半导体激光联合全蝎软膏可有效促进大鼠DSU创面的愈合,减轻炎症反应并修复血管功能,效果优于全蝎软膏组。

Objective:To explore the repairing effect of semiconductor laser combined with scorpion ointment on diabetic skin ulcer(DSU)in rats.Methods:24 SPF Wistar rats were randomly divided into control group,model group,scorpion ointment group and combined group,DSU model was established by intraperitoneal injection of SZT-skin defect.After modeling,DSU rats were treated with laser and/or scorpion ointment respectively.Body weight,blood glucose and wound granulation tissue healing were observed on the 3 d,7 d,10 d and 14 d.Subsequently,the H.E staining,immunohistochemistry,ELISA and Western blot were used to detect the wound tissue.Results:Compared with the control group,the body weight and blood glucose of DSU rats were significantly different(P<0.05),and the modeling rate was 73.33%.The wound healing rate of each treatment group was higher than that of the model group(P<0.05),of which the granulation tissue grows vigorously,capillaries are abundant,and inflammation is reduced.The results of ELISA and Western blot showed that compared with the model group,the VEGF in the combined group increased significantly(P<0.05),while the TNF-αand Smad 4 protein were significantly decreased(P<0.05).The therapeutic effect was better than that of scorpion ointment group.Conclusions:Semiconductor laser combined with scorpion ointment can effectively promote the wound healing of DSU in rats,reduce the inflammatory reaction and repair the vascular function,which effect is better than that of scorpion ointment group.