呼出气冷凝液收集检测技术在临床中的研究进展

Research Advance in clinical study of exhaled breath condensate collection technology

呼出气冷凝液(exhaled breath condensate,EBC)生化指标检测是一种反映下呼吸道生化状态的新方法,可用于评价气道炎症和氧化应激程度,又称为生化肺功能。文章总结了临床中常用的EBC收集装置,包括呈规模化的商业化收集装置(如R-Tube、EcoScreen、TURBO-DECCS、ANACON)以及自制的EBC收集装置;EBC常用的检测指标,如NO及其衍生物、花生四烯酸衍生物、H2O2、细胞因子、pH值等;EBC的敏感性与准确性以及EBC收集的影响因素。EBC作为一种非侵入性评价肺功能指标的检测技术,因其无创、简便等优点,有望成为肺功能监测的重要组成部分,从而为机械通气期间肺功能保护提供新的监测方法。

The detection of biochemical indicators by exhaled breath condensate(EBC)is a new method to reflect the lower respiratory tract lining.It can be used to evaluate the degree of airway inflammation and oxidative stress,also known as biochemical pulmonary function.This review describes the exhaled breath condensate collection device commonly used in the clinic,including large-scale commercial collection devices(such as R-Tube,EcoScreen,TURBO-DECCS and ANACON)and self-made EBC collection devices,commonly used test indicators for EBC,such as NO and its derivatives,arachidonic acid derivatives,H2O2,cytokines,pH and so on.This review also discussed the sensitivity and accuracy of EBC compared to traditional invasive detection methods and the factors affecting EBC collection.As a non-invasive evaluation technique for lung function,EBC is expected to be an important part of pulmonary function monitoring because of its non-invasive and simple advantages.Thus,it provides a new monitoring method for lung function protection during mechanical ventilation.