德国小蠊室内人工饲养技术研究

Study on indoor rearing technique of Blattella germanica

目的提高和更新德国小蠊室内标准化饲养技术,便于获得大量标准试虫,为防治德国小蠊卫生杀虫剂的筛选和相关科学研究提供参考。方法在温度(27±1)℃,湿度(60±10)%,无光照条件(除操作时间)下,连续饲养德国小蠊1年,以德国小蠊的卵期、若虫期、产卵前期、世代以及雌雄成虫体质量为评价指标,检验德国小蠊各阶段的发育历期及雌雄成虫体质量,评价饲养技术的可靠性。结果德国小蠊各发育阶段的历期为卵期(15.75±1.26)、若虫期(30.50±1.29)、产卵前期(9.00±0.82)和世代(55.25±1.71)d,单雄成虫重(54.36±0.19)mg,单雌成虫重(104.78±3.09)mg。结论使用该饲养方法,德国小蠊发育历期缩短,各阶段生长发育一致性较好,适于德国小蠊的周年继代,可作为德国小蠊新的标准化的饲养程序。

Objective To improve and update the standard indoor rearing technique of Blattella germanica for an easy access to a large number of standard test insects, and to provide a reference for the screening of sanitary insecticides against B. germanica and conducting relevant research. Methods The cockroaches were reared under a continuous dark condition(except operating time) at(27±1) ℃ and(60±10)% humidity for one year. The duration of each developmental stage(egg stage, nymphal stage, pre-oviposition period, and generation time) and the body weights of male and female adults were tested to evaluate the reliability of this rearing technique. Results The developmental time of the cockroaches was(15.75±1.26) d for the egg stage,(30.50±1.29) d for the nymphal stage,(9.00±0.82) d for the pre-oviposition period, and(55.25±1.71) d for the generation time. A male adult weighed(54.36±0.19) mg, and a female adult weighed(104.78±3.09) mg. Conclusion This rearing method shortens the developmental periods of B. germanica, and makes a good consistency of growth and development for all stages. It is suitable for the annual generation of B. germanica and can be used as a new standard indoor rearing technique.