Study on Extraction Method of Carotenoids from Citrus Leaves

柑橘叶片类胡萝卜素提取方法研究

In order to explore the extraction method of carotenoids from citrus leaves,the extraction effect of carotenoids from leaves of Honey pomelo by ultrasonic-assisted extraction was investigated by single factor experiment and orthogonal experiment.The results showed that the optimum extraction condition was:the ration of ethanol and acetone 1∶1(v/v,containing 0.1%BHT),solid-liquid ratio 1∶30(g/mL),extraction time 7 min,ultrasonic power 240 W,extraction frequency 3 times.Under the above condition,the extraction rate of carotenoids was 85.98%.

为了探究柑橘叶片类胡萝卜素提取方法,通过单因素试验和正交试验考察超声波辅助法提取三红蜜柚叶片中类胡萝卜素的效果。结果表明:最佳提取方法为提取剂选用乙醇∶丙酮=1∶1(v/v,含0.1%BHT),料液比1∶30(g/mL),提取时间7 min,超声波功率240 W,提取次数3次。在此提取工艺条件下,得到柑橘叶片类胡萝卜素的提取率为85.98%。