响应面法优化太子参蒸汽爆破提取多糖的工艺研究

Optimization of Steam Explosion Process Condition for Extracting Polysaccharides from Pseudostellaria heterophylla by Response Surface Methodology

【目的】优化蒸汽爆破技术提取太子参多糖的工艺条件。【方法】以太子参为基质材料,采用Box-Behnken设计分析蒸汽压强、稳压时间和基质含水量3个因素对多糖提取含量的影响。【结果】结果表明,各因素均显著影响试验指标;太子参多糖最佳提取工艺条件为蒸汽压强1.50 Mpa、稳压时间46 s和基质含水量46%,在此条件下,多糖含量的试验验证值为39.32%,与模型预测值相对标准偏差为2.73%。扫描电镜结果也表明优化条件组其表面结构破碎无序、有裂痕,有效改变和破坏了太子参的细胞层次物理结构。【结论】本研究为太子参多糖新的提取工艺提供一种可行性思路,也为其资源产业化应用提供了参考。

【Objective】To optimize the steam explosion process condition for extracting polysaccharides from Pseudostellaria heterophylla.【Method】The effects of steam pressure,pressure-maintaining time and material moisture content on the extraction of polysaccharides from Pseudostellaria heterophylla were studied by response surface methodology based on Box-Behnken design.【Result】The findings showed that each factor could significantly affect the test index,and the optimum condition was as follows:steam pressure 1.50 Mpa,pressuremaintaining time 46 s and material moisture content 46%.Under this condition,the verified experimental value of polysaccharides from Pseudostellaria heterophylla was 39.32%,indicating a relative standard deviation of 2.73%from the predictive value.Meanwhile,scanning electron microcopy(SEM)images showed that the surface physical structure of Pseudostellaria heterophylla was irregularly broken and cracked,which means the physical structure of Pseudostellaria heterophylla was changed and destroyed at the cellular level.【Conclusion】This experiment provides a new approach for the extraction of polysaccharides from Pseudostellaria heterophylla,as well as a reference for the resource utilization of Pseudostellaria heterophylla.