γ射线辐照对食用菌基质中微生物的影响

Effects ofγIrradiation on Microorganisms in Edible Fungi Matrix

以食用菌基质中微生物为研究对象,研究γ射线辐照处理对基质中微生物数量及群落的影响。结果表明:基质中菌落总数和霉菌数经过γ射线辐照后显著减少;γ射线辐照对基质中细菌种水平OTU和属水平OTU相对丰度影响较大;种水平上,Escherichia-Shigella、Uncultured bacterium(Lactococcus)、Uncultured organism 3条细菌OTU的丰度在辐照后明显上升,其余细菌OTU明显降低;属水平上,辐照对Escherichia-Shigella影响较小;而从门水平上来看辐照对菌群影响不大,Proteobacteria和Firmicutes两个门的细菌始终是优势菌群。γ射线辐照对基质中真菌的菌群结构影响较小,属水平上,Fusatium、Unclassified、Candida、Aspergillus在辐照前后始终是优势菌群;门水平上,Ascomycota是主要优势菌。

The microbial quantity and community of edible fungi substrate were studied.The results showed that the total number of colonies and molds in the substrate decreased significantly afterγirradiation.γirradiation had obvious effects on relative abundance of bacteria OTU at species level.The OTU abundances of Escherichia-Shigella,Uncultured bacterium(Lactococcus),Uncultured organism significantly increased after the irradiation,whereas the remaining bacteria OTU decreased at species level.γirradiation had no obvious effects on relative abundance of Escherichia-Shigella at genus level and also had no obvious effects on relative abundance of bacteria at gate level,Proteobacteria and Firmicutes were always the dominant flora.Irradiation had little effects on the flora structure of fungi in the substrate.In terms of genus level,Fusatium,unclassified,Candida and Aspergillus were always the dominant flora before and after irradiation.In terms of phylum level,Ascomycota was the dominant flora.