西葫芦环斑病病原鉴定及葫芦科作物对西瓜花叶病毒抗性筛选

Identification of pathogen causing zucchini ringspot disease and screening of resis-tant varieties

西瓜花叶病毒(watermelon mosaic virus,WMV)是危害我国葫芦科作物生产的重要病原。培育和种植抗病品种是防治病毒病最经济有效的措施。本研究利用RT-PCR和血清学方法在表现环斑症状的西葫芦果实上检测到WMV,未检测到番木瓜环斑病毒(papaya ringspot virus,PRSV)。接种WMV侵染性克隆到西葫芦品种绿源冬宝,果实表面产生环斑症状,表明WMV是引起西葫芦果实表面环斑症状的重要病原。利用间接ELISA方法对山东泰安的西瓜、黄瓜、甜瓜、西葫芦和南瓜等46个样品检测发现33个样品表现WMV血清学阳性(检出率71.74%),薛庄采集的带有环斑症状的西葫芦均检测到WMV。对山东省81份葫芦科作物种质资源抗WMV鉴定发现西葫芦品种万盛丰宝、盛丰金珠表现中抗(MR);西瓜品种绿宝新秀和浪潮一号表现中抗(MR);甜瓜品种黄皮面瓜表现抗病(R);黄瓜品种星君贝贝表现中抗(MR);南瓜品种爱维80南瓜表现高抗(HR),蜜本南瓜、传统蜜本南瓜、七叶早南瓜、玲珑二号和绿贝贝迷你南瓜等5个品种表现抗病(R);瓠瓜品种均表现为感病(S)。研究结果对合理布局葫芦科作物品种防控WMV具有重要意义。

Watermelon mosaic virus(WMV;genus Potyvirus)is an important viral pathogen infecting cucurbit crops in China.Breeding and planting resistant varieties are the most economical and effective strategy to control viral disease.In this study,WMV but not papaya ringspot virus(PRSV)was detected on fruits of zucchini exhibiting ringspot symptoms by RT-PCR and virus specific antibodies.Zucchini cultivar‘LvYuanDongBao'plants inoculated with WMV infectious clone pCamWMV-GFP showed ringspots on fruits,suggesting WMV is an important pathogen causing ringspot symptoms on zucchini fruits.In total,46 samples collected from watermelon,cucumber,melons,zucchini and pumpkin plants in Tai'an,Shandong,were tested by WMV specific antibody using Plate Trapped Antibody-Enzyme Linked Immuno Sorbent Assay(PTA-ELISA).Results showed that 33 samples were WMV positive(71.74%).Zucchini fruits displaying ringspot symptoms from Xuezhuang were all WMV positive.Resistance to WMV in 81 cucurbit crops germplasm resources from Shandong Province was identified.Results showed that zucchini cultivars‘Wanshengfengbao'and‘Shengfengjinzhu'were moderately resistant to WMV;watermelon cultivars‘Lvbaoxinxiu'and‘Langchao 1'were moderately resistant to WMV;melon cultivar‘Huangpimian melon'was resistant to WMV;cucumber cultivar‘Xingjun beibei'were moderately resistant to WMV;pumpkin cultivar‘Aiwei 80 pumpkin'was highly resistant,and five cultivars including‘Miben pumpkin',‘traditional Miben pumpkin',‘Qiye early pumpkin',‘Linglong 2'and‘Green beibei mini pumpkin'showed resistance to WMV.All tested bottle gourd cultivars were susceptible or highly susceptible to WMV.These results will be helpful to control WMV by utilizing resistance of different cucurbit crops.