阴沟肠杆菌MY01的鉴定及防治水稻细菌性条斑病研究

Identification of Enterobacter cloacae MY01 and prevention of bacterial leaf streak

肠杆菌属(Enterobacter sp.)作为有益微生物的重要菌种,不仅对一些植物的生长起着促进作用,而且在有害生物防治方面起着重要的作用。本研究采用Biolog微生物生理生化分析、共培养等手段,对阴沟肠杆菌MY01、抗生素溶杆菌13-6与水稻细菌性条斑病菌RS105的营养和空间竞争能力、定殖特性以及对水稻幼苗的促生作用、条斑病的生防效果进行测定,明确阴沟肠杆菌与抗生素溶杆菌对水稻条斑病的协同生防作用。结果显示MY01生长迅速且能利用大部分碳源,营养竞争能力较强,可限制其他细菌生长。温室喷雾接种时,MY01与13-6对水稻条斑病的防效相近,且MY01可增强13-6对水稻条斑病的相对防效,防效高达93.44%。阴沟肠杆菌MY01作为一种潜在的生防菌株,对水稻条斑病具有一定防效,且促进抗生素溶杆菌13-6对水稻条斑病的防治。

As beneficial microorganisms,Enterobacter sp.not only promotes the growth of some plants,but also plays an important role in the control of harmful organisms.In this study,Biolog microbial physiological and biochemical analysis,co-culture and other means were used to study the nutritional and spatial competitiveness,colonization characteristics and the promoting effect on rice seedlings of Enterobacter cloacae MY01,Lysobacter antibioticus 13-6 and Xanthomonas oryzae pv.oryzicola RS105.The biocontrol effect of stripe disease was determined to determine the synergistic effect of E.cloacae and L.antibioticus on bacterial leaf streak(BLS).The results show that MY01 grows rapidly and can utilize most of the carbon and nitrogen sources,so it has strong nutritional competitiveness and limits the growth of other bacteria.When inoculated in the greenhouse,the control effect of MY01 on BLS is similar as 13-6 and MY01can enhance the relative control effect of 13-6 on BLS,and the control effect can reach 93.44%.As a potential strain,E.cloacae MY01 has certain control effects on BLS and promotes the prevention and controlof L.antibioticus 13-6 against BLS.