摘要
目的明确TRIM26在人和小鼠不同组织器官中的表达定位和水平。方法表达定位研究采用免疫组织化学PV-9000二步法(非生物素),利用Bio GPS数据库分析TRIM26在人体组织中的表达水平,RT-qPCR检测小鼠主要组织器官中TRIM26表达水平。结果IHC的结果显示,TRIM26蛋白信号定位于细胞质和细胞核,其表达多见于上皮细胞。TRIM26蛋白在人和小鼠的食管鳞状上皮细胞、胃上皮细胞、小肠上皮细胞、结肠上皮细胞、胰岛细胞、脑胶质细胞、肾小管上皮细胞和肾小球细胞以及子宫内膜腺上皮细胞中的表达定位一致。另外,TRIM26分别在人体气管组织和小鼠脾组织上的相对表达量最高。结论TRIM26在人和小鼠组织上的表达定位具有一致性,表达量略有差异。TRIM26在人和小鼠主要组织器官上的表达谱的明确,将为利用TRIM26条件性敲除小鼠解析TRIM26在不同组织器官的功能奠定了良好基础。
Objective To examine the expression level and distribution of TRIM26 in multiple human and mouse tissues and organs.Methods The immunohistochemistry(IHC)PV-9000 two-step method(non-biotin)was used to determine the expression and distribution of TRIM26.BioGPS was used to calculate TRIM26 expression levels in human tissues and organs,whereas RT-qPCR was performed to determine its counterparts in mice.Results The IHC result indicated that the TRIM26 protein is expressed in the cytoplasm and nucleus,mainly in epithelial cells.Its expression distribution in human esophageal squamous cells,gastric epithelial cells,small intestinal epithelial cells,colon epithelial cells,islet cells,brain glial cells,renal tubular epithelial cells,glomerular cells,and endometrial glandular epithelial cells was consistent with that in the mouse equivalent cells.The highest relative expression of TRIM26 was observed in human tracheal cells and mouse spleen.Conclusions Humans and mice show consistent expression distribution of TRIM26 in multiple tissues and organs,but with different expression levels.Revealing the TRIM26 expression profile in human and mouse main organs and tissues lays a foundation for further exploration of the functions of TRIM26 in different tissues and organs by engineering conditional TRIM26-knockout mice.
作者
廉梅
林晓琳
贾俊双
肖胜军
肖东
LIAN Mei;LIN Xiaolin;JIA Junshuang;XIAO Shengjun;XIAO Dong(School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515;China.2.Laboratory Animal Management Center,Southern Medical University,Guangzhou 510515;.3.Pathology Department of the Second Affiliated Hospital of Guilin Medical University,Guilin 541199)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第8期10-15,共6页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金项目(81702778)
桂林市科学研究与技术开发计划课题(20130120-8)
广西自然科学基金项目(2020GXNSFAA159131)。