MPTP诱导C57BL/6小鼠帕金森模型的制备和评估

Preparation and assessment of a C57BL/6 mouse Parkinson’s model induced by MPTP

目的建立稳定的1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导C57BL/6小鼠帕金森模型,并对模型进行行为学评估。方法建立给药组,给药剂量分别为35 mg/kg、30 mg/kg、25 mg/kg和给予30 mg/kg生理盐水的对照组。分别进行震颤麻痹评分、旷场实验、爬杆实验、悬挂实验、游泳实验,并进行综合统计。分析给药组与对照组间以及各给药组间的差异。同时进行HE和免疫组化TH抗体染色,观察黑质和纹状体区域神经元和神经纤维的改变。结果与对照组相比,给药组评分均明显增高,但25 mg/kg剂量组行为学评分明显低于35 mg/kg和30 mg/kg剂量组,35 mg/kg与30 mg/kg剂量组间无显著性差异。黑质区域TH阳性神经元数量减少,纹状体TH阳性神经纤维减少。结论建立了一个可以通过行为学数据评估小鼠帕金森病模型效果的简便途径。

Objective To establish a stable Parkinson’s model in C57BL/6 mice by MPTP and assess behavioral scores of the model.Methods Dosing groups(35,30,and 25 mg/kg body weight MPTP)and control group(30 mg/kg body weight physiological saline)were established.We conducted the paralysis agitans score,open field test,pole test,traction test,swim test,and comprehensive statistical analysis.We then analyzed the differences between each group and the control group.HE staining and TH immunohistochemical staining were performed to observe the substantia nigra,striatum neurons,and nerve fibers.Results Compared with the control group,the scores of drug administration groups were significantly higher.The ethology score of the 25 mg/kg dose group was significantly lower than those of 35 and 30 mg/kg groups.No significant difference was found between 35 mg/kg and 30 mg/kg groups.Substantia nigra TH-positive neurons and striatum TH-positive nerve fibers were decreased.Conclusions A simple method was established to evaluate a Parkinson’s disease model in mice by behavioral data.