间苯三酚对体外受精-胚胎移植结局的影响

Effects of phloroglucinol on transfer outcome of in vitro fertilization-embryo transfer

目的探究间苯三酚对体外受精-胚胎移植(IVF-ET)结局的影响。方法回顾性分析2015年1月1日至2018年11月30日期间在郑州大学第二附属医院生殖医学部行IVF-ET治疗的4289例不孕患者的临床资料。根据妊娠结局分为妊娠组及非妊娠组,分析影响患者妊娠结局的危险因素。再根据移植前用药情况将患者分为未接受药物治疗组(A组)、术前静脉滴注阿托西班组(B组)、术前联合术中注射阿托西班组(C组)、术中静脉滴注间苯三酚组(D组),比较四组患者的一般情况、妊娠结局,以及治疗期间及治疗后不良反应发生情况。结果年龄、优质胚胎数及移植前用药方式是影响IVF-ET患者妊娠结局的独立因素(P=0.031、P=0.039、P=0.020)。D组患者胚胎种植率、生化妊娠率及临床妊娠率均明显高于A组(P均<0.001)、B组(P均<0.001)及C组(P<0.001,P<0.001,P=0.001);其异位妊娠率明显低于A组、B组及C组(P均<0.001);流产率低于A组(P<0.001)。治疗期间及治疗后,4组患者均未见不适主述及过敏反应,所有出生婴儿均无出生缺陷。结论用药方式是影响IVF-ET患者妊娠结局的独立因素,术中静脉注射间苯三酚可明显提升IVF-ET患者胚胎种植率及妊娠率,并降低其流产率。

Objective To explore the effects of phloroglucinol on transfer outcome of in vitro fertilization-embryo transfer(IVF-ET).Methods Retrospective analysis of 4289 cases of infertility patients treated with IVF-ET in the Department of Reproductive Medicine,the Second Affiliated Hospital of Zhengzhou University from January 1,2015 to November 30,2018 was performed.According to the pregnancy outcome,all patients were divided into pregnancy group and non-pregnancy group to analyze the risk factors affecting the pregnancy outcome.According to medication before transfer,all patients were divided into no medication group(group A),preoperative intravenous infusion of atosiban group(group B),preoperative and intraoperative injection of atosiban group(group C)and interoperative intravenous infusion of phloroglucinol group(group D).The pregnancy outcomes,occurrence of adverse reactions during and after treatment were compared among the four groups.Results Age,number of high-quality embryos,and medication methods before transfer were independent factors influencing pregnancy outcomes in patients undergoing IVF-ET(P=0.031,P=0.039,P=0.020).The embryo implantation rate,the biochemical pregnancy rate and the clinical pregnancy rate of group D were significantly higher than those in group A(all P<0.001),group B(all P<0.001)and group C(P<0.001,P<0.001,P=0.001),while the ectopic pregnancy rate was significantly lower than that in group A,group B and group C(all P<0.001).The abortion rate in group D was lower than that in group A(P<0.001).During treatment period and after treatment,there were no discomfort complains and allergic reactions in the four groups.There were no birth defects in all born babies.Conclusion The medication method is an independent factor influencing pregnancy outcomes of patients undergoing IVF-ET.Intraoperative intravenous infusion of phloroglucinol can significantly increase embryo implantation rate and pregnancy rate of IVF-ET patients,and reduce their abortion rate.