壳聚糖修饰Fe3O4纳米颗粒的制备及其磁共振造影性能

Preparation of FeO Nanoparticles Modified With Chitosan and Magnetic Resonance Imaging Performance

采用碳二亚胺法将壳聚糖(CS)分子链上的—NH2和羧基化Fe3O4(Fe3O4-COOH)纳米粒子表面的—COOH共价耦联,制备壳聚糖修饰的Fe3O4(CS-Fe3O4)纳米颗粒。通过纳米粒度仪、透射电子显微镜(TEM)、傅里叶变换红外(FT-IR)光谱仪、X-射线衍射(XRD)分析仪、低温综合物性测试系统分别对CS-Fe3O4纳米颗粒的粒径分布、表面形态、化学结构、晶体结构及磁学性质进行测试,并研究其作为T2造影剂的体外磁共振造影能力。结果表明,制备的CS-Fe3O4纳米颗粒形态圆整、大小均一,水合动力学平均粒径为109.2 nm,具有良好的分散稳定性及超顺磁性;体外磁共振显像实验表明该纳米粒子具有负性显像能力,并且其负性显像能力随Fe3O4浓度增加而增强。因此,CS-Fe3O4纳米颗粒有望成为一种新型的磁共振T2造影剂。

Chitosan modified Fe3O4(CS-Fe3O4)nanoparticles were prepared by coupling—NH2 on the molecular chains of chitosan(CS)with—COOH covalently on the surface of carboxylated Fe3O4(Fe3O4-COOH)nanoparticles by carbon diimine method.The size distribution,surface morphology,chemical structure,crystal structure and magnetic property of CS-Fe3O4 nanoparticles were characterized by nanometer particle size analyzer(Zetasizer),transmission electron microscope(TEM),fourier transform infrared(FT-IR)spectrometer,X-ray diffractometer(XRD),and low temperature comprehensive physical property test system,and their vitro magnetic resonance imaging(MRI)ability as a T2 contrast agent was also explored.The results showed that the prepared CS-Fe3O4 nanoparticles with a hydrodynamic average size of 109.2 nm were round in shape and uniform in size,showing good dispersion stability and superparamagnetism.Vitro magnetic resonance imaging experiment showed that the nanoparticles had negative imaging ability,and its negative imaging ability increased with the increase of Fe3O4 concentration.Thus,CS-Fe3O4 nanoparticles are expected to be a novel magnetic resonance T2 contrast agent.