病毒感染抑郁症患者血清IFN-γ和IL-6变化及CXCL10/CXCR3作用

Changes of IFN-γ,IL-6 and role of CXCL10/CXCR3 in depression patients with viral infection

目的探讨病毒感染抑郁症患者血清干扰素-γ(IFN-γ)、白细胞介素-6(IL-6)水平变化及CXC趋化因子配体10(CXCL10)/CXC趋化因子受体3(CXCR3)通路作用。方法选取2017年1月-2018年12月武汉市精神卫生中心收治的抑郁症患者200例,根据是否发生病毒感染将其分为感染组(n=102)和未感染组(n=98),另选100名同期健康志愿者设置为对照组。酶联免疫法检测血清中IFN-γ、IL-6水平及CXCL10/CXCR3表达,并使用逆转录聚合酶链反应(RT-PCR)检测其mRNA水平。结果感染组以艾滋病病毒(HIV)、乙型肝炎病毒(HBV)、爱泼斯坦巴尔病毒(EBV)感染为主。感染组患者血清IFN-γ、IL-6、CXCL10、CXCR3水平高于未感染组和对照组(P<0.05),且未感染组高于对照组(P<0.05)。感染组CXCL10、CXCR3 mRNA水平高于未感染组和对照组(P<0.05),且未感染组高于对照组。受试者工作特征曲线(ROC)分析结果显示,血清IFN-γ、IL-6和CXCL10/CXCR3对抑郁症患者病毒感染具有一定的临床诊断价值(P<0.05),且三指标联合诊断价值更高(P<0.05)。结论 IFN-γ、IL-6和CXCL10/CXCR3信号通路对预测抑郁症患者病毒感染具有重要的临床价值,其免疫学机制有待进一步研究。

OBJECTIVE To explore the changes of levels of serum interferon-γ(IFN-γ)and interleukin-6(IL-6)and observe the pathway effect of CXC chemokine ligand 10(CXCL10)/CXC chemokine receptor 3(CXCR3).METHODS A total of 200 depression patients who were treated in the Wuhan Mental Health Center from Jan 2017 to Dec 2018 were enrolled in the study and divided into the infection group with 102 cases and the non-infection group with 98 cases according to the status of viral infection,meanwhile,100 healthy volunteers were chosen as the control group.The levels of serum IFN-γand IL-6 and expression of CXCL10/CXCR3 were detected by using enzyme-linked immunosorbent assay,and the mRNA of the study objects was determined by means of reverse transcription polymerase chain reaction(RT-PCR).RESULTS The patients with HIV,HBV and EBV infections were dominant in the infection group.The levels of serum IFN-γ,IL-6,CXCL10 and CXCR3 of the infection group were significantly higher than those of the non-infection group and the control group(P<0.05),and the levels of above indexes of the non-infection group were significantly higher than those of the control group(P<0.05).The levels of CXCL10 and CXCR3 mRNA of the infection group were significantly higher than those of the non-infection group and the control group(P<0.05),and the levels of above indexes of the non-infection group were significantly higher than those of the control group.The result of receiver operating characteristic(ROC)curve analysis showed that the serum IFN-γ,IL-6 and CXCL10/CXCR3 had significant clinical value in diagnosis of viral infection in the depression patients(P<0.05),and the diagnostic value of the joint detection of the three indexes was significantly higher(P<0.05).CONCLUSION The IFN-γ,IL-6 and CXCL10/CXCR3 signaling pathways have significant clinical value in prediction of viral infection in the depression patients,and the immunological mechanisms need to be further studied.