骨骼肌钝挫伤兔模型制备与打击力度的关系

Preparing a blunt contusion model of rabbit skeletal muscle under different blow strengths

背景:骨骼肌钝挫伤模型的研究多以大鼠为实验对象,作者认为以兔为实验对象可以更好地观察其宏观及影像学变化。目的:以自制重物下砸仪为实验基础,根据不同打击高度,制备骨骼肌损伤兔模型,希望为后续兔腓肠肌钝挫伤模型复制提供参考数据。方法:33只新西兰兔随机分为4组,正常组3只,75 cm高度打击组、50 cm高度打击组、25 cm高度打击组各10只,除正常组外均进行不同力度的打击,制备骨骼肌损伤兔模型。通过大体观察、苏木精-伊红染色、Masson染色、超声影像等结果对比不同高度打击后的骨骼肌损伤程度。结果与结论:①从50 cm高度进行打击,可对骨骼肌造成中度损伤,其自然恢复时间大于21 d,适合于骨骼肌钝挫伤模型的建立;②75 cm高度打击易造成骨折,动物死亡较多;③25 cm高度打击,损伤较轻,7 d即可恢复,不适合用于骨骼肌损伤模型的建立。

BACKGROUND:In current studies regarding skeletal muscle blunt contusion model,rats are often taken as the experimental object.The authors believe that rabbits are the better experimental object to observe the macroscopic and imaging changes after blunt contusion.OBJECTIVE:Based on the self-made heavy-duty smashing instrument as the experimental basis,to replicate the skeletal muscle injury model according to different strike heights,which is expected to provide a reference basis for the subsequent replication of the rabbit gastrocnemius blunt contusion model.METHODS:Thirty-three New Zealand rabbits were randomly divided into four groups:normal group(n=3),75 cm strike group(n=10),50 cm strike group(n=10),25 cm strike group(n=10).Except for the normal group,all strikes were performed with different strengths.The severity of skeletal muscle damage after different strikes was compared through gross observation,hematoxylin-eosin staining,Masson staining,and ultrasound imaging.RESULTS AND CONCLUSION:The 50 cm height blow can cause moderate damage to the skeletal muscle,and the natural recovery time is over 21 days,which is suitable for the establishment of skeletal muscle blunt contusion model.The 75 cm height blow is likely to cause fractures,resulting in more deaths.The 25 cm height blow can cause mild damage that can be recovered within 7 days,which is not suitable for the establishment of skeletal muscle injury model.