洛阳市2416例女性人乳头瘤病毒感染情况及基因亚型分析

Prevalence and genotyping of human papillomavirus infection in 2416 women from Luoyang

目的分析洛阳地区人乳头瘤病毒(HPV)患者的年龄分布、HPV感染模式、易感亚型,为该区域HPV感染流行病学研究及宫颈癌的防治提供参考依据。方法应用荧光定量聚合酶链式反应(PCR),对2019年5-11月前来洛阳市妇幼保健院妇科就医送检的2416份宫颈脱落细胞标本,进行21种HPV基因分型检测。结果受检的2416例女性中,有495例感染HPV,感染率为20.49%。其中,60岁以上年龄段女性感染率最高为32.43%,第二位是51~60岁年龄段,感染率为28.76%,第三位是<30岁年龄段,感染率为21.31%。HPV单一感染358例,感染率为72.32%,多重感染137例,感染率为27.68%,最多可检出六重感染。21种基因亚型均有检出,其中高危型HPV l6、HPV 52、HPV53、HPV 58、HPV39为检出率最高的5种感染亚型。结论HPV感染趋向低龄化,HPV感染率与年龄呈“U”形分布。感染模式以单一感染最常见。HPV l6、HPV52、HPV53、HPV58、HPV39为最常见HPV感染亚型。HPV感染情况和基因亚型具有地域特征。

Objective To investigate the age distribution,infection pattern and susceptible genotypes of human papillomavius(HPV)infected patients in Luoyang,so as to provide the reference basis for the epidemiological study of HPV infection and the prevention and treatment of cervical cancer in this areas.Methods Fluorescence quantitative polymerase chain reaction(PCR),was used to detect 21 kinds of HPV genotypes in 2416 cervical exfoliated cell samples from May 2019 to November 2019.Results Of the 2416 cases,495 were infected with HPV,with an infection rate of 20.49%.Among them,the infection rate of women over 60 years old was the highest(32.43%).Followed by the infection rate of 51-60 group(28.76%),and third was the infection rate of younger than 30 group(21.31%).Single HPV infection and multiple HPV infection were observed in 358 cases and 137 cases,with the infection rate was 72.32%and 27.68%,respectively.Up to six infections can be detected.21 genotypes were detected,and HPVl6、HPV 52、HPV53、HPV58、HPV39 were the five genotypes with the highest detection rates.Conclusion The HPV infection tends to infect the younger females,and the study shows a“U”shaped distribution of age-specific prevalence of HPV.The most common pattern is the single infection.The most common four genotypes of HPV infection are HPVl6,HPV52,HPV53,HPV58 and HPV39.Prevalence and genotyping of HPV infection have regional characteristics.