不同酶免检测试剂检测抗HIV的结果比较

Comparison of the results of different enzyme-linked immunoassay reagents in the detection of anti HIV

目的比较在抗艾滋病病毒(HIV)检测中采用不同酶免检测试剂的诊断结果。方法选择我院收诊的120例疑似HIV感染患者,分别采用第三代、第四代酶免检测试剂对HIV抗体进行检测。以蛋白印迹试验结果为金标准,比较第三代与第四代酶免检测试剂对HIV感染的诊断灵敏度、特异度、准确度、阳性预测值、阴性预测值,分析第三代、第四代酶免检测试剂诊断结果与蛋白印迹试验诊断结果之间的一致性。结果第四代酶免检测试剂对HIV感染的诊断灵敏度、特异度、准确度、阳性预测值、阴性预测值均高于第三代酶免检测试剂(P<0.05)。第四代酶免检测试剂诊断结果与蛋白印迹试验诊断结果之间具有高度一致性(Kappa=0.749),而第三代酶免检测试剂诊断结果与蛋白印迹试验诊断结果之间具有中度一致性(Kappa=0.561)。结论第四代酶免检测试剂在HIV抗体检测中的诊断价值良好,可对HIV感染进行灵敏、准确地诊断,可作为HIV感染诊断中采用酶联免疫吸附法检测时的主要试剂。

Objective To compare the diagnostic results of different enzyme-linked immunoassay reagents in the detection of anti human immunodeficiency virus(HIV). Methods A total of 120 patients with suspected HIV infection in our hospital were selected, and the third and fourth generation enzyme immunoassay reagents were used to detect HIV antibody respectively.Western blot assay was used as the gold standard, and the sensitivity, specificity, accuracy, positive predictive value and negative predictive value of the third and fourth generation enzyme immunoassay reagents for HIV infection were compared. The consistency between the diagnosis results of the third and fourth generation enzyme immunoassay reagents and Western blot assay were analyzed. Results The sensitivity, specificity, accuracy, positive predictive value and negative predictive value of the fourth generation enzyme immunoassay reagent were higher than those of the third generation enzyme immunoassay reagent(P <0.05). The diagnostic result of the fourth generation enzyme immunoassay reagent was highly consistent with that of Western blot assay(Kappa = 0.749), while the diagnostic result of the third generation enzyme immunoassay reagent was moderately consistent with that of Western blot assay(Kappa = 0.561). Conclusion The fourth generation enzyme immunoassay reagent has a good diagnostic value in HIV antibody detection, it can be used for sensitive and accurate diagnosis of HIV infection, and can be used as the main reagent in the diagnosis of HIV infection by enzyme-linked immunosorbent assay.