西达本胺对NK细胞体外杀伤K562细胞活性的影响及其相关机制

Effect of Chidamide on the Killing Acitivity of NK Cells Targeting K562 Cells and Its Related Mechanism In Vitro

目的:观察西达本胺对自然杀伤细胞(NK)体外杀伤K562细胞活性的影响并探讨其作用机制。方法:用不同浓度的西达本胺预处理K562细胞,与NK细胞不同效靶比共同孵育,采用流式细胞术检测西达本胺作用于K562细胞后NK细胞对其杀伤作用、K562细胞表面NKG2D相关配体表达量及K562细胞凋亡率的变化。结果:西达本胺可增加K562细胞对NK细胞杀伤的敏感性,同时可上调K562细胞表面ULBP2表达,对ULBP1、MICA/MICB表达无明显影响,西达本胺于K562细胞本身无明显细胞毒性作用。结论:西达本胺可能通过增加ULBP2的表达提高K562细胞对NK细胞杀伤敏感性。

Objective:To investigate the effect of chidamide on the killing activity of NK(Natural killer cell,NK)cells targeting K562 cells and its related mechanism.Methods:K562 cells were pretreated with chidamide at different concentrations and cocultured with NK cells at different effect-target ratios.The killing effect of chidamide on K562 cells by NK cells,the expression of natural killer group 2 member D(NKG2D)ligands and apoptosis rate of K562 cells were detected by flow cytometry.Results:The killing sensitivity of NK cells to K562 cells could be enhanced by chidamide.The expression of ULBP2 on K562 cell surface could be up-regulate,however,the expression of ULBP1 and MICA/MICB showed no statistically difference as compared with control group.Chidamide showed no obvious cytotoxicity to K562 cells.Conclusion:Chidamide can significantly improve killing efficiency of NK cells on K562 cells,which may be related to the up-regulation of ULBP2 expression.