多功能蛋白聚糖及其相关分子在多发性骨髓瘤患者中的表达及其临床意义

Expression of Versican and Its Related Molecules in Patient with Multiple Myeloma and Its Clinical Significance

目的:探讨多功能蛋白聚糖及其相关分子在多发性骨髓瘤(MM)患者中的表达及其临床意义。方法:采用Ficoll密度梯度离心法分离25例多发性骨髓瘤患者治疗前、后骨髓单个核细胞,并通过实时定量PCR检测细胞中VCAN及其相关分子m RNA的表达水平,采用Western blot法测定其蛋白表达。结果:MM患者治疗有效后,VCAN、FAK、FN的表达量较治疗前均明显下降(P<0.05),而MK及HAS治疗前、后差异无统计学意义(P>0.05);未缓解组VCAN、FAK、FN、MK、HAS的表达量较正常对照组均明显升高(P<0.05),未缓解组FAK、FN较初诊组表达量均明显升高(P<0.05);VCAN mRNA相对表达量在ISS分期Ⅲ期较正常对照组显著升高(P<0.05),较Ⅰ期明显升高(P<0.05),但较Ⅱ期无明显差异(P>0.05)。Spearman相关分析结果显示,初诊MM患者骨髓单个核细胞中VCAN与FAK(r=0.69)、MK(r=0.67)、FN(r=0.67)的相对表达水平均呈正相关(P<0.05),而VCAN与HAS相关性无统计学意义(r=0.259,P>0.05)。生存分析结果显示,VCAN mRNA相对表达水平与患者的总生存期(OS)(P<0.05)及无进展生存期(PFS)(P<0.05)相关。结论:VCAN及其相关分子在多发性骨髓瘤患者骨髓单个核细胞中高表达,其在多发性骨髓瘤的发生、发展及预后中可能起重要作用。

Objective:To investigate the expression and clinical significant of VCAN and its related molecules in patients with MM.Methods:Ficoll density gradient centrifugation method was used to speared the bone marrow mononuclear cell in 25 cases of MM before and after treatment,the relative mRNA expression of VCAN and their related molecules(FAK,FN,MK,and HAS)in bone marrow was detected by real-time quantitative PCR,and their protein expression was determined by Western bolt.Results:The expression of VCAN,FK and FN in the effective group after treatment was significantly lower than that before treatment(P<0.05),however,the expression of MK and HAS showed no statistically significantly different before and after treatment(P<0.05).The expression of VCAN of patients in non remission group was significantly higher than that in control group(P<0.05).The expression of FAK and FN of patients in no remission group was significant increased as compared with the patients in newly diagnosed group(P<0.05).The relative expression of VCAN mRNA in the patients at 3rd stage was significantly higher than those at the 1st stage(P<0.05)and control group but showed no significant difference to the patients at 2nd stage(P<0.05).The expression of VCAN and its related proteins(FAK,MK,FN)showed positively correlation in bone marrow mononuclear cells of MM patients(P<0.05).The correlation between VCAN and HAS was not statistically significant(r=0.259,P>0.05).Survival analysis showed that the relative expression of VCAN mRNA was associated with OS(P=0.049)and PFS(P=0.041)in MM patients.Conclusion:VCAN and its related molecules are highly expressed in MM patients;VCAN may act as potential biomarker in the development of multiple myeloma.