环状RNA circ_0024707对乳腺癌细胞的生长和转移的影响

Effects of Circular RNA circ_0024707 on the Growth and Metastasis of Breast Cancer Cells

目的环状RNA(circRNA)hsa_circ_0024707尚未被报道研究。本研究通过检测hsa_circ_0024707在乳腺癌组织和细胞株中的表达,探索环状RNA hsa_circ_0024707通过调节miR-448对乳腺癌细胞增殖和侵袭的影响。方法实时定量聚合酶链反应(qPCR)检测乳腺癌组织利细胞株中hsa_circ_0024707的表达。以hsa_circ_0024707表达最低的乳腺癌细胞株感染空载慢病毒(对照组)和载有hsa_circ_0024707的慢病毒(实验组)。CCK-8实验和Transwell侵袭实验检测hsa_circ_0024707过表达对乳腺癌细胞株增殖和侵袭的影响。生物信息学技术预测hsa_circ_0024707的下游基因。qPCR和蛋白免疫印迹(Western blot)法检测下游基因的表达。结果hsa_cire_0024707在乳腺癌组织和癌旁组织中表达分別为1.73±0.46和4.53±0.76(P<0.05)。与永生化乳腺上皮细胞比较,hsa_circ_0024707在乳腺癌细胞株中呈低表达(P<0.05),在BT549细胞中的表达量最低(P<0.01)。与对照组比较,过表达hsa_cire_0024707可抑制BT549细胞的增殖(P<0.05)。对照组和实验组侵袭细胞数分別为82.46±8.49和38.34±8.84(P<0.05)o hsa_circ_0024707靶基因可能为miR-448,miR-448靶基因可能为SPRY2。与对照组比较,hsa_cire_0024707过表达导致BT549细胞中miR-448的表达下调(P<0.01),SPRY2的表达上调(P<0.01),Wnt/β-catenin信号通路被抑制。结论hsa_circ_0024707在乳腺癌组织和细胞株中均呈低表达,hsa_circ_0024707能抑制乳腺癌BT549细胞的增殖和侵袭,其分子机制可能是下调miR-448的表达,上调SPBY2蛋白的表达,抑制Wnt/β-catenin信号通路的活化.

Objective The cireular RNA(eircRNA)hsa_eire_0024707 has not been reported.To analyze the expression of eireular RNA(cireRNA)hsa.cire.0024707 in breast cancer tissues and cell lines,and to explore the efct of circular RNA hsa_circ.0024707 on the proliferation and invasion of breast cancer cells by regulating miR-448.Methods Real-time quantitative polymerase chain reaction(qPCR)was used to detect the expression of hsa_eirc_0024707 in breast cancer tissues and cell lines.The breast cancer cell line expressing lowest hsa_cire_0024707 was infected with empty lentivirus(control group)and lentivirus carrying hsa_circ.0024707(experi-mental group).CCK-8 assay and Transwell invasion assay were used to detect the effect of hsa_eire_0024707 overexpression on proliferation and invasion of breast cancer cell lines.Bioinformatics technology predicts downstream genes of hsa_circ._0024707.qPCR and West-ern blot were used to detect the expression of downstream genes.Results The expression of hsa_cire_0024707 in breast cancer tissues and adjacent issues was 1.73+0.46 and 4.53+0.76(P<0.05).Compared with immortalized mammary epithelial cells,hsa_eire_0024707 was down-regulated in breast cancer cell lines(P<0.05),and lowest in BT549 cells(P<0.01).Compared with the control group.overexpression of hsa_cire_0024707 inbibited the proliferation of BT549 cells(P<0.05).The number of invasive cells in the control group and the experimental group were 82.46+8.49 and 38.34±8.84.respectively(P<0.05).The hsa_cire_0024707 target gene may be miR-448 and the miR-448 target gene may be SPRY2.Compared with the control group,overexpression of hsa_cire_0024707 resulted in down-regulation of miR-448 expression in BT549 cells(P<0.01),up-regulation of SPRY2 expression(P<0.01),and inhibition of Wnt/β-catenin signaling pathway.Conclusion hsa_cire.0024707 is lowly expressed in breast cancer tissues and cell lines.hsa_cire.0024707 can inhibit the proliferation and invasion of breast cancer BT549 cells.The molecular mechanism may be to down-regulate the expression of miR-448,up-regulate the expression of SPRY2 protein and inhibition of activation of Wnt/β-catenin signaling pathway.