培本固疏方对去势大鼠骨代谢及骨生物力学的影响

Effect of Peiben Gushu Decoction on bone metabolism and bone biomechanics in ovariectomized rats

目的观察培本固疏方对去卵巢骨质疏松模型大鼠骨密度、骨生物力学及骨代谢指标的影响。方法将42只SD大鼠随机分为假手术组(SHAM)、模型对照组(OVX)、中药低剂量组(D)、中药中剂量组(Z)、中药高剂量组(G)和西药组(X),通过切除大鼠双侧卵巢建立骨质疏松症模型,假手术组仅切除卵巢周围约1 g的脂肪组织。造模4周后进行药物干预,各组持续灌胃8周。计算子宫指数,测定股骨骨密度(bone mineral density,BMD)及股骨生物力学弹性载荷、极限载荷、弹性模量等指标;采用ELISA法测定血清骨代谢指标TRACP5b、sRANKL、OPG及氧化应激指标MDA、AGEs的含量。结果与SHAM组比较,OVX组子宫指数、股骨近端和股骨中段BMD、弹性载荷、弹性模量均明显降低(P<0.05),OVX组血清MDA、AGEs含量明显升高(P<0.01),血清TRACP5b含量明显升高(P<0.01),而OPG、OPG/sRANKL比值下降(P<0.01);与OVX组相比,各治疗组子宫指数、骨强度均有所改善。Z组、G组及X组BMD均明显增高(P<0.05)。Z组、G组及X组TRACP5b含量下降(P<0.05)、OPG含量升高(P<0.05)、RANKL含量降低、OPG/sRANKL比值升高(P<0.05)。Z组、G组血清MDA、AGEs含量降低(P<0.05)。结论培本固疏方可以增加骨强度,通过OPG/RANKL/RANK通路来抑制破骨细胞形成,减缓骨基质吸收,抗氧化应激机制也可能参与其中,具体机制有待探讨。

Objective To observe the effects of Peiben Gushu Decoction on bone mineral density,bone biomechanics and bone metabolism in ovariectomized osteoporosis model rats.Methods 42 SD rats were randomly divided into sham operation group(SHAM),model control group(OVX),Chinese medicine low-dose group(D),Chinese medicine middle-dose group(Z),Chinese medicine high-dose group(G)and western medicine group(X),the osteoporosis model was established by removing bilateral ovaries of rats,and the sham group only removed about 1 g of adipose tissue around the ovaries.Drug intervention was performed 4 weeks after modeling,and each group was continuously administered for 8 weeks.The uterus index was calculated,and the femoral bone density and femoral biomechanical elastic load,ultimate load,elastic modulus and other indicators were determined.The serum bone metabolism indexes TRACP5b,sRANKL,OPG and oxidative stress indicators MDA and AGEs were determined by ELISA.Results Compared with the SHAM operation group,the uterus index,BMD of the proximal femur and BMD,elastic load and elastic modulus of the femur were significantly decreased(P<0.05).The serum MDA and AGEs levels in the model group were significantly increased(P<0.01),the serum TRACP5b content increased significantly(P<0.01),while the OPG,OPG/sRANKL ratio decreased(P<0.01);compared with the model group:the uterus index and bone strength of each treatment group improved.The BMD of Peiben Gushu Decoction,high dose group and Alendronate group were significantly increased(P<0.05).The content of TRACP5b and OPG was decreased(P<0.05),The content of RANKL and OPG/sRANKL ratio were increased(P<0.05).The serum levels of MDA and AGEs in the high-dose group were significantly lower(P<0.05).Conclusion Peiben Gushu Decoction can increase bone strength,inhibit osteoclast formation through OPG/RANKL/RANK pathway,slow bone matrix uptake,and anti-oxidative stress mechanism may also be involved.The specific mechanism remains to be explored.