氯化锂对骨质疏松大鼠股骨干骨折愈合早期影响的研究

Effect of lithium chloride on the early healing of femoral shaft fractures in osteoporotic rats

目的探讨氯化锂对骨质疏松大鼠股骨干骨折愈合早期的影响。方法将20只24月龄老年Sprague-Dawley大鼠随机分为对照组和治疗组,每组10只。所有大鼠均在右侧股骨干中段制作骨折模型,并用克氏针固定。在实验过程中,治疗组接受200 mg/(kg·d)的氯化锂治疗。术后4周后,检测血清Ⅰ型胶原氨基端延长肽(P1NP)和β-I型胶原羧基端肽(CTX)水平;使用Micro-CT和组织学以及生物力学评估骨折愈合情况。结果与对照组相比,术后4周治疗组大鼠血清P1NP和CTX有明显改善(P均<0.05);与治疗组相比,在骨折后4周,Micro-CT和组织学及生物力学评估显示对照组的骨折愈合延迟,具有较差骨痂微观结构和强度。氯化锂治疗可以显著改善骨折愈合延迟。结论氯化锂干预可以促进老年骨质疏松大鼠股骨干骨折早期愈合。

Objective To explore the early effects of lithium chloride on the healing of femoral shaft fractures in aged osteoporosis rats.Methods Twenty elderly osteoporotic Sprague-Dawley rats were randomly divided into two groups,the control group and the treatment group,with 10 rats in each group.All the rats were fractured in the middle of the right femoral shaft and fixed with a Kirschner wire.During the experiment,the treatment group received 200 mg/(kg·d) of lithium chloride.Four weeks after surgery,serum type Ⅰ collagen amino terminal prolongation peptide(P1 NP) and β-type Ⅰ collagen carboxy terminal peptide(CTX) levels were measured;fracture healing was assessed using Micro-CT and histology and biomechanics.Results Compared with the control group,the serum P1 NP and CTX in the treatment group were significantly improved 4 weeks after operation(P<0.05).Compared with the treatment group,Micro-CT,histology and biomechanical evaluation showed that the fracture healing in the control group was delayed And had poor microstructure and strength at 4 weeks after fracture.Lithium chloride therapy can significantly improve these changes.Conclusion Lithium chloride intervention can promote the early healing of femoral shaft fracture in elderly osteoporotic rats.