胃癌组织中ZMYND8蛋白的表达及临床病理学意义

Expression of ZMYND8 Protein in Gastric Cancer and Its Clinicopathological Significance

目的检测溴区结合蛋白ZMYND8在胃癌组织及相应癌旁组织中的表达,明确ZMYND8的表达水平与胃癌患者临床病理学参数之间的关系,并分析ZMYND8表达水平与胃癌患者5年生存率之间的相关性,以期明确其在判断胃癌患者预后中的作用。方法采用实时荧光定量PCR法(RT-q PCR)检测ZMYND8基因mRNA在30例胃癌及癌旁新鲜组织中的表达;采用免疫组织化学SABC法检测ZMYND8蛋白在60例肿瘤及癌旁石蜡标本中的表达;ZMYND8蛋白表达水平与胃癌患者生存率之间的相关性采用K-M生存曲线分析。结果胃癌组织中ZMYND8 mRNA表达显著低于相对应的癌旁组织(P<0.01);ZMYND8蛋白在胃癌组织中表达的阳性率为21.6%,显著低于癌旁中的85.0%(P<0.05)。ZMYND8的表达与肿瘤细胞浸润深度、淋巴结转移及TNM分期密切相关(P<0.05),而与患者年龄、性别、肿瘤分化程度、远处转移无显著相关性(P>0.05)。K-M生存分析结果表明,癌组织中ZMYND8表达阳性的患者5年生存率显著高于阴性表达组,差异具有统计学意义(P<0.01)。结论ZMYND8在胃癌组织中表达率低,且与肿瘤浸润、淋巴结转移、TNM分期及患者5年生存率密切相关,提示检测该基因的表达对预测胃癌患者预后具有重要意义。

Objective To detect the expression of ZMYND8 in gastric cancer tissuesand their adjacent tissues, clarify the relationship between the expression level of ZMYND8 and the clinicopathological parameters of gastric cancer patients, and analyze the relationship between the expression of ZMYND8 and the 5-year survival rate of gastric cancer patients. Methods The expression of ZMYND8 mRNA in 30 cases of gastric cancer fresh tissues and the adjacent tissues were detected by RTq PCR. Immunohistochemical was used to detect the expression of ZMYND8 protein in 60 cases of tumor and paraffinic samples. The correlation between the ZMYND8 expression and the survival rate of patients was analyzed by K-M survival curve. Results The expression of ZMYND8 mRNA in gastric cancer was significantly lower than that in the adjacent tissues(P<0.01). The positive rate of ZYND8 protein expression in gastric cancer was 21.6%, which was significantly lower than that in the adjacent tissues(85.0%, P<0.05). ZMYND8 expression was closely related to the depth of tumor cell invasion, lymph node metastasis and TNM stage(P<0.05). The results of K-M survival analysis showed that the 5-year survival rate of patients with positive expression of ZMYND8 was significantly higher than that of patients with low expression(P<0.01). Conclusion The expression rate of ZMYND8 in gastric cancer is low and closely related to tumor invasion, lymph node metastasis, TNM stage and 5-year survival rate of patients, which suggest that detection of the expression of ZMYND8 is of great significance in predicting the prognosis of gastric cancer patients.