高尿酸血症及并其发症大鼠模型的建立

Establishment of a rat model of hyperuricemia and its complications

目的建立一种稳定高效的高尿酸血症合并肝肾损伤及痛风性关节炎等并发症的大鼠模型。方法雄性Sprague-Dawley大鼠(下称SD大鼠) 48只,随机分为6组(每组8只),分别为空白对照组(Control组)、氧嗪酸钾组(OA组)、氧嗪酸钾+次黄嘌呤组(OA+H组)、氧嗪酸钾+酵母膏组(OA+YE组)、10%果糖+0.2%乙胺丁醇组(10%Fru+0.2%EMB组)、2%氧嗪酸钾联合12%酵母特殊饲料组(OA PO组)五种不同药物与不同浓度的实验,5周后通过大鼠血清尿酸、肌酐、尿素等生化指标的测定以及肾和关节滑膜肌病理切片观察。结果与空白对照组比较,OA PO组血清尿酸值升高,伴有严重的肝肾损伤和关节炎症。OA、OA+H、OA+YE、10%Fru+0.2%EMB组未达到高尿酸水平,但出现一定程度的肝肾损伤及关节炎症。结论 2%氧嗪酸钾和12%酵母膏混合饲料建立高尿酸血症及并发症模型的效果最佳,此模型也适用于高尿酸血症引起肾损及关节炎症的相关研究。

Objective To establish an efficient rat model of hyperuricemia with liver and kidney injury,gouty arthritis and other complications. Methods Forty-eight male Sprague-Dawley rats were randomly divided into the blank control group( control group),oteracil potassium group( OA group),oteracil potassium with 6-hydroxypurine group( OA+ H group),oteracil potassium with yeast extract group( OA + YE group),10% fructose with 0. 2% ethambutol group( 10% Fru + 0. 2% EMB group),and the 2% oteracil potassium mixed with 12% yeast extract peculiar feed group( OA PO group)( n = 8 rats per group). Blood was collected from the tail vein once weekly,the serum uric acid and urea values were detected,and the model was studied for 5 consecutive weeks. Results Compared with the control group,the OA PO group reached high serum uric acid levels with severe liver and kidney damage and joint inflammation in week 5. The uric acid values of the OA + H,OA + YE and 10% Fru + 0. 2% EMB groups failed to reach high levels. However,biochemical indicators showed that liver and kidney damage occurred in these four groups. Hematoxylin and eosin staining showed that the knee joints of the model group had synovial thickening and scattered and disordered arrangement to varying degrees.Conclusions The 2% oteracil potassium mixed with 12% yeast extract diet is the best diet for establishing a rat model of hyperuricemia and its complications and is suitable for studying hyperuricemia-induced renal injury and arthritis.