深度水解蛋白的无乳糖奶粉在无菌大鼠培育中的应用研究

Applications for deep hydrolyzed protein-lactose-free milk powder in germ-free rat cultivation

目的无菌大鼠的培育中,出生后饲喂的人工配制乳对于无菌大鼠的培养至关重要。深度水解蛋白的无乳糖奶粉,便于婴幼儿吸收,减少了各种消化不良和过敏反应,那么在无菌大鼠使用的奶中加入这种奶粉是否会对无菌大鼠的培育有积极的作用,并且检测饲养成功的无菌大鼠的生化指标。方法使用清洁级的SD大鼠,确定临产期,摘除子宫后,在隔离器内剥离大鼠幼崽,使用人工方法哺乳至22 d离乳,记录体重和生存率。使用的配方奶中,加入深度水解蛋白无乳糖奶粉的为实验组,对照组中加入全价配方奶粉。对培育成功的无菌大鼠在第8周时检测血生化指标。结果在人工喂养的前14天,实验组与对照组在体重和生存率上都无差别,从第14天开始实验组的体重和生存率开始高于对照组,直至第22天实验组的生存率与对照组相比极显著提高(37.18%vs17.78%),第22天的体重也显著高于对照组(9.96±0.49) vs(13.36±0.59)。无菌大鼠的血生化指标显示AST(天门冬氨酸氨基转移酶)降低,GLU(葡萄糖)升高。结论在培育无菌大鼠幼鼠阶段的过程中,深度水解蛋白的无乳糖奶粉可以作为配方奶成分的重要的成分进行添加,可以有效的增加幼鼠体重,减少死亡率。雌性无菌大鼠的血生化指标变动较大。

Objective Artificially formulated milk fed after birth is essential for cultivating germ-free rats.Lactose-free milk powder with deep hydrolyzed protein is easily absorbed by infants and reduces indigestion and allergic reactions. We detected the biochemical indexes of germ-free rats to investigate whether adding lactose-free milk powder to their milk would have a positive effect on the rats. Methods Pregnant Sprague-Dawley rats were determined to be in labor.After surgically removing the uterus,the young rats were housed in isolators and artificially lactated for 22 days. Body weight and survival rates were recorded. The experimental group received lactose-free milk powder with deep hydrolyzed protein in their milk,and the control group received regular formula milk. The blood biochemical indexes were measured at8 weeks. Results During the first 14 days of artificial feeding,body weight and survival rates did not differ between the experimental and control groups. The weights and survival rates of the experimental group began to increase compared with those of the control group on day 14. The survival rate and body weights of the experimental group were significantly higher than those of the control group on day 22 [37. 18% vs 17. 78% and( 9. 96 ± 0. 49) g vs( 13. 36 ± 0. 59) g,respectively]. Blood biochemical indexes of the germ-free rats showed that their aspartate aminotransferase levels decreased,and their glucose levels increased. Conclusions In cultivating germ-free rat pups,lactose-free milk powder with deep hydrolyzed protein can be added to formula milk to effectively increase the weight of the young rats and reduce their mortality rate. Adding lactose-free milk powder to formula milk substantially changed the blood biochemical indexes of female germ-free rats.