燕麦馒头对高脂小鼠降血脂、抗氧化及保护肝脏作用的研究

Effects of Oat Steamed Bread on the hypolipidemic,Antioxidation and liver protection in Hyperlipidemia Mice

研究燕麦发酵前后对高血脂小鼠血脂、抗氧化以及肝保护作用。雄性KM小鼠随机分为4组,每组12只,分别为正常对照组、高脂模型组、燕麦面条组、燕麦馒头组。6周后检测血清、肝脏中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平及抗氧化丙二醛(MDA)、超氧化物歧化酶(SOD)、还原谷胱甘肽(GSH)指标,同时观察肝组织损伤变化情况。燕麦馒头组小鼠血清及肝脏中TG、TC、LDL-C水平均显著(P<0.05)或极显著(P<0.01)降低,HDL-C水平显著升高(P<0.05);与高脂模型组比较,肝脏中TC、TG、LDL-C分别下降46.43%、50%、45.95%,HDL-C上升32.08%,血清中TC、TG、LDL-C分别下降19.59%、30.81%、17.45%,HDL-C上升24.34%,燕麦馒头降低血脂效果在肝脏中更加明显。燕麦馒头组和燕麦面条组小鼠肝脏、血清中的SOD和GSH活性均显著(P<0.05)或极显著(P<0.01)升高,MDA含量显著(p<0.05)或极显著(P<0.01)降低,说明燕麦发酵前后均增强抗氧化能力,且发酵后抗氧化能力明显高于发酵前。病理学检测结果显示燕麦馒头具有减少高脂血症小鼠肝脏中脂质沉积的作用。燕麦发酵后对高血脂小鼠有显著降低血脂、抗氧化及降低肝损伤作用。

Objective:The protective effect of oat steamed bread on hypolipidemic,antioxidant and liver protection in hyperlipidemia mice were investigated.Methods:Male KM mice were randomly divided into 4 groups:normal control group,high-fat model group,oat noodle group and oat steamed bread group.The levels of lipids(TC,TG,HDL-C,LDL-C)and antioxidants(MDA,SOD and GSH)were measured in the serum and liver,and the changes of liver injury were observed after 6 weeks.Results:The serum and liver levels of TG,TC and LDL-C in the oat steamed bread group were significantly decreased(P<0.05)or significantly decreased(P<0.01),while the levels of HDL-C were significantly increased(P<0.05).Compared with the high-fat model group,the content of TC,TG and LDL-C in the liver decreased by 46.43%,50%and 45.95%,HDL-C increased by 32.08%.And the content of TC,TG and LDL-C in the serum decreased by 19.59%,30.81%and 17.45%,HDL-C increased by 24.34%,respectively.The serum and liver levels of SOD and GSH in the oat steamed bread and oat noodle group were significantly increased(P<0.05)or significantly increased(P<0.01),while the levels of MDA were significantly decreased(P<0.05)or significantly decreased(P<0.01).It showed that the antioxidant capacity of oat was enhanced after fermentation.At the same time,the results of pathological examination showed that the oat steamed bread group had the effect of reducing the lipid deposition in the liver of hyperlipidemia mice.Conclusion:Whole powder oatmeal steamed bread can have a significant role in reducing blood lipid,antioxidation and liver protection in hyperlipidemia mice.