阿特拉津降解菌W11的分离鉴定及降解特性研究

Isolation,Identification and Characterization of Atrazine-Degrading Bacterial Strain W11

分离、筛选出降解阿特拉津残留的高效功能菌株,对于农产品安全和土壤环境修复具有重要的现实意义。通过富集培养法,从吉林省延吉市的农田土壤中分离出1株以阿特拉津为唯一氮源生长的降解菌株W11。经生理生化和16S rRNA基因序列分析,鉴定W11为类节杆菌属(Paenarthrobacter sp.)。菌株W11在无机盐液体培养基培养60 h对阿特拉津(100 mg/L)的降解率为97.1%,其适宜温度范围为25℃~35℃,最适温度为30℃;适宜的酸碱度范围为pH值6~9,最适pH值为7。最佳碳源是葡萄糖,外加氮源对菌株降解阿特拉津的能力几乎无影响,菌株W11在修复阿特拉津污染方面具有很好的应用前景。

Isolation and screening of highly efficient functional strains capable of degrading atrazine is of great practical significance for agricultural product safety and soil environmental remediation.In this study,a degrading strain W11 was isolated from the surface soil of maize field by enrichment method,which could use atrazine as a sole nitrogen source to grow.Based on its biochemical characterization and 16 S rRNA gene sequence,strain W11 was identified as Paenarthrobacter sp.It could degrade 97.1%of atrazine(100 mg/L)in the medium within 60 h.Its optimum temperature and pH range for growth were 25℃-35℃and 6-9,respectively;Its optimum temperature and pH were 30℃and 7,respectively.Glucose was the most suitable carbon source for its growth and atrazine degradation.Additional nitrogen sources did not affect the degradation of atrazine.Strain W11 has a great potential for bioremediation of atrazine-contaminated soil.