丙泊酚通过下调miR-135b表达抑制乳腺癌MDA-MB-231细胞增殖、侵袭和迁移的研究

Propofol inhibits proliferation,invasion and migration of breast cancer MDA-MB-231 cells by down-regulating the expression of miR-135b

目的研究丙泊酚对乳腺癌MDA-MB-231细胞增殖、侵袭和迁移情况的影响。方法体外培养乳腺癌MDA-MB-231细胞,并随机分为7组,空白组不给予任何处置;阴性对照组给予0.1%二甲亚砜处置;低、中、高剂量实验组分别给予25,50和100μmol·L^-1丙泊酚处置;转染组和阴性转染组分别转染miR-135b mimics及miR-135b mimics-NC,并添加100μmol·L^-1丙泊酚处置。用逆转录聚合酶链反应检测细胞miR-135b的表达情况,用CCK-8法检测细胞的增殖情况,用划痕实验检测细胞迁移情况,用Transwell实验检测细胞的侵袭情况。结果干预48 h后,阴性对照组、转染组和中、高剂量实验组的miR-135b表达量分别为(0.94±0.02),(0.91±0.05),(0.71±0.02)和(0.40±0.01),增殖率分别为(94.27±3.12)%,(99.99±3.05)%,(58.67±3.14)%和(42.15±4.34)%,细胞迁移率分别为(82.12±3.49)%,(84.03±3.16)%,(31.87±3.61)%和(17.38±2.54)%,侵袭细胞数分别为(610.57±18.24),(615.72±9.63),(371.44±13.44)和(201.46±9.48)个,高剂量实验组上述指标与阴性对照组和转染组比较,差异均有统计学意义(均P<0.05)。结论丙泊酚可能通过抑制miR-135b的表达,从而抑制乳腺癌MDA-MB-231细胞的增殖、迁移及侵袭。

Objective To study the effects of propofol on proliferation,invasion and migration of breast cancer MDA-MB-231 cells.Methods Breast cancer MDA-MB-231 cells were cultured in vitro,and divided into seven groups.Blank control group was not given any treatment.Negative control group was given 0.1% dimethylsulfoxide.Experimental-L,-M and-H groups were treated with 25,50 and 100μmol·L^-1 propofol.The transfection and negative transfection group were transfected with miR-135b mimics and miR-135b mimics-NC respectively,and then treated with 100μmol·L^-1 propofol.The expression of miR-135b was detected by reverse transcription-polymerase chain reaction.CCK-8 method was used to detect cell proliferation.Scratch test was used to detect cell migration.Transwell assay was used to detect cell invasion.Results After administration 48 h,the main indexes of negative control group,transfection group,experimental-M group and experimental-H group were compared:the expressions of miR-135b were(0.94±0.02),(0.91±0.05),(0.71±0.02)and(0.40±0.01),the proliferation rates were(94.27±3.12)%,(99.99±3.05)%,(58.67±3.14)% and(42.15±4.34)%,cell mobilities were(82.12±3.49)%,(84.03±3.16)%,(31.87±3.61)%and(17.38±2.54)%,the number of invasion cells were(610.57±18.24),(615.72±9.63),(371.44±13.44)and(201.46±9.48),respectively.The above indexes in the experimental-H group were significantly different from those in the negative control group and transfection group(all P<0.05).Conclusion Propofol may inhibit the proliferation,migration and invasion of breast cancer MDA-MB-231 cells by inhibiting the expression of miR-135b.