丝氨酸/精氨酸蛋白特异性激酶-2在胃癌组织中的表达情况及临床意义

Expression and clinical significance of serine/arginine protein-specific kinase 2 in gastric cancer

目的探讨丝氨酸/精氨酸蛋白特异性激酶-2(SRPK2)在胃癌组织中的表达情况与胃癌患者临床特征及预后的关系。方法收集胃癌患者经手术切除的胃癌组织及相应的癌旁组织标本各85例,采用免疫组织化学法检测SRPK2在85例胃癌患者胃癌组织及癌旁组织中的表达情况,分析胃癌组织中SRPK2阳性表达与胃癌患者临床特征及预后的关系。结果免疫组化染色结果显示,胃癌组织中SRPK2的阳性表达率明显高于癌旁组织(P<0.01)。浸润深度(根据T分期)为T3~4期、有淋巴结转移、肿瘤直径>5cm、TNM分期为Ⅲ~Ⅳ期胃癌患者胃癌组织中SRPK2的阳性表达率均高于浸润深度(根据T分期)为T1~2期、无淋巴结转移、肿瘤直径≤5cm、TNM分期为Ⅰ~Ⅱ期的胃癌患者,差异均有统计学意义(P<0.05);不同性别、年龄、肿瘤分化程度、肿瘤部位胃癌患者胃癌组织中SRPK2的阳性表达率比较,差异均无统计学意义(P>0.05)。Kaplan-Meier生存曲线显示,SRPK2阳性表达的胃癌患者的术后5年总生存率低于SRPK2阴性表达的胃癌患者(P<0.05)。结论SRPK2在胃癌组织中的阳性表达率较高,可能与胃癌的发生、发展有关。SRPK2阳性表达的胃癌患者的预后较差,其可能成为胃癌临床诊疗及预后评估的生物学标志物。

Objective To investigate the expression of serine/arginine protein-specific kinase 2(SRPK2) in gastric cancer and analyze the correlation between SRPK2 and the clinical characteristics and prognosis.Method A total of 85 specimens of cancer tissue and corresponding gastric mucosa tissue were collected.The expression of SRPK2 was detected by immunohistochemistry,and the relationship between SRPK2 and the clinical characteristics and prognosis of gastric cancer was analyzed.Result Immunohistochemical staining showed that the positive expression rate of SRPK2 in gastric cancer was statistically higher than that in adjacent tissues(P<0.05).The positive expression rate of SRPK2 was higher in patients with T3-4 invasion depth(according to T stage),lymph node metastasis,tumor diameter>5 cm,TNM stage Ⅲ-Ⅳ gastric cancer than those with invasion depth(according to T stage) T1-2,no lymph node metastasis,tumor diameter≤5 cm,TNM stage Ⅰ-Ⅱ,the difference was statistically significant(P<0.05).There was no significant difference in the positive expression rate of SRPK2 in different gender,age,tumor differentiation degree and tumor site(P>0.05).Kaplan-Meier survival curve showed that the five-year survival rate of gastric cancer patients in SRPK2 positive group was significantly lower than that in SRPK2 negative group(P<0.05).Conclusion The increased expression of SRPK2 in gastric cancer may be related to the occurrence,development,invasion and metastasis of gastric cancer.The prognosis of SRPK2 positive patients is poor,which may become a biological marker of clinical diagnosis and prognosis evaluation of gastric cancer.