miR-126、miR-96与急性心肌梗死患者血小板活化及近期预后相关性

Correlation of miR-126 and miR-96 with platelet activation and short-term prognosis in patients with acute myocardial infarction

目的探究miR-126、miR-96与急性心肌梗死(AMI)患者血小板活化、近期预后相关性。方法选取2018年1月至2019年9月本院126例行急诊冠脉造影且确诊为AMI患者作为研究组,收集89例冠状动脉造影正常的非AMI患者作为对照组。统计两组miR-126、miR-96水平,不同miR-126、miR-96水平患者血小板活化指标[血小板活化依赖性颗粒表面膜蛋白(CD62p)、血小板膜糖蛋白Ⅱb/Ⅲa复合物纤维蛋白原受体(PAC-1)、血小板活化因子(PAF)]、90 d不良心血管事件(MACE)发生率,分析miR-126、miR-96与血小板活化指标、MACE发生率关系。结果研究组miR-126水平低于对照组,miR-96水平高于对照组,差异有统计学意义(P<0.05);低miR-126、高miR-96患者CD62p、PAC-1、PAF水平及90 d MACE发生率高于高miR-126、低miR-96患者,差异有统计学意义(P<0.05);Spearman分析,CD62p、PAC-1、PAF及90 d MACE发生率与miR-126呈负相关,与miR-96呈正相关,差异有统计学意义(P<0.05)。结论AMI患者外周血miR-126、miR-96水平显著升高,且与血小板活化、近期预后相关性好,可作为鉴别诊断AMI标志物,指导临床治疗。

Objective To investigate the correlation between miR-126 and miR-96 and platelet activation and short-term prognosis in patients with acute myocardial infarction(AMI).Methods From January 2018 to September 2019,126 patients undergoing emergency coronary angiography and diagnosed with AMI in our hospital were selected as the study group,89 non-AMI patients with normal coronary angiography were collected as the control group.The levels of miR-126 and miR-96,platelet activation indicators in patients with different levels of miR-126 and miR-96[platelet activation-dependent granule surface membrane protein(CD62 p),platelet membrane glycoproteinⅡb/Ⅲa complex fibrinogen receptor(PAC-1),platelet activating factor(PAF)],the incidence of adverse cardiovascular events(MACE)at 90 days were counted in two groups,the relationship between miR-126,miR-96,platelet activation index and incidence of MACE was analyzed.Results The level of miR-126 in the study group was lower than that in the control group,and the level of miR-96 was higher than the control group(P<0.05).The levels of CD62 p,PAC-1,PAF and incidence of MACE at 90 days in patients with low miR-126 and high miR-96 were higher than those with high miR-126 and low miR-96(P<0.05).Spearman analysis showed that the incidence of CD62 p,PAC-1,PAF and incidence of MACE at 90 days was negatively correlated with miR-126 and positively correlated with miR-96(P<0.05).Conclusion The levels of miR-126 and miR-96 in peripheral blood of patients with AMI are significantly increased,and they have a good correlation with platelet activation and short-term prognosis,which can be used as a differential diagnosis marker for AMI and guide clinical treatment.