ST6GalⅠ抑制TNFR1表达调控肾透明细胞癌凋亡

ST6GalⅠinhibits the expression of TNFR1 to regulate the apoptosis of renal clear cell carcinoma

目的探讨ST6GalⅠ对肾透明细胞癌细胞凋亡的影响及可能的作用机制。方法qRT-PCR和Western blot检测ST6GalⅠ和TNFR1在肾透明细胞癌细胞中的表达。构建ST6GalⅠ差异表达肾透明细胞癌细胞株,采用流式细胞术检测细胞凋亡水平,并分析ST6GalⅠ和TNFR1表达的相关性,qRT-PCR和Western blot检测差异表达ST6GalⅠ细胞中TNFR1的表达,采用流式细胞术检测同时差异表达ST6GalⅠ和TNFR1的A498和Caki-1细胞的凋亡水平。结果与ST6GalⅠ在5种肾透明细胞癌细胞中均高表达(P<0.05);抑制ST6GalⅠ表达后A498细胞凋亡率升高(P<0.01),Caki-1细胞过表达ST6GalⅠ后细胞凋亡率降低(P<0.001)。TNFR1 mRNA和蛋白在5种肾透明细胞癌细胞株中均低表达,且与ST6GalⅠm RNA和蛋白表达呈负相关(r=-0.9667,-0.9928,均P<0.01);抑制ST6GalⅠ表达TNFR1表达增加(P<0.001),而ST6GalⅠ过表达TNFR1表达降低(P<0.001);A498细胞同时抑制ST6GalⅠ和TNFR1表达后细胞凋亡率低于单独抑制ST6GalⅠ(P<0.01),Caki-1细胞同时过表达ST6GalⅠ和TNFR1后细胞凋亡率高于单独抑制ST6GalⅠ(P<0.01)。结论ST6GalⅠ可通过抑制TNFR1表达而抑制肾透明细胞癌的凋亡。

Objective To investigate the effect of ST6GalⅠon the apoptosis of renal clear cell carcinoma cells and its possible mechanism.Methods The expression of ST6GalⅠand TNFR1 in renal clear cell carcinoma cell lines were detected by qRT-PCR and Western blot.The renal clear cell carcinoma cell lines with different expression of ST6GalⅠwere constructed,and the apoptosis level was detected by flow cytometry,and the correlation between the expression of ST6GalⅠand TNFR1 was analyzed,the expression of TNFR1 in the differentially expressed ST6GalⅠcells were detected by qRT-PCR and Western blot,and the apoptosis level of A498 and Caki-1 cells that differentially expressed ST6GalⅠand TNFR1 were detected by flow cytometry.Results ST6GalⅠwas highly expressed in 5 renal clear cell carcinoma cell lines(P<0.05).The apoptosis rate of A498 cells increased after ST6GalⅠwas inhibited(P<0.01),while the apoptosis rate of Caki-1 cells reduced for the overexpression of ST6GalⅠ(P<0.001).TNFR1 mRNA and protein were low-expressed in 5 renal clear cell cancer cell lines,and negatively correlated with ST6GalⅠmRNA and protein expression(r=-0.9667,-0.9928,all P<0.01).The expression of TNFR1 increased when the expression of ST6GalⅠ(P<0.001)was inhibited,while the expression of TNFR1 decreased for the overexpression of ST6GalⅠ(P<0.001).The apoptosis rate of A498 cells after inhibition of both ST6GalⅠand TNFR1 expression was lower than that of ST6GalⅠalone(P<0.01),and the apoptosis rate of Caki-1 cells after overexpression of both ST6GalⅠand TNFR1 was higher than that of ST6GalⅠalone(P<0.01).Conclusions ST6GalⅠcan inhibit the apoptosis of renal clear cell carcinoma cell lines by inhibiting the expression of TNFR1.