NEDD4基因多态性与瘢痕妊娠关联性研究

Study on the association between NEDD4 gene polymorphism and scar pregnancy

目的研究NEDD4基因多态性与瘢痕妊娠的关联性。方法选择2018-01~2019-09在该院就诊的40例孕妇为研究对象,根据超声结果分为瘢痕妊娠组和非瘢痕妊娠组,每组20例。抽取肘静脉血,提取DNA样本。对NEDD4基因的rs2271289、rs2303579、rs10518830位点进行测序。比较两组基因型及等位基因频率。结果rs2271289位点的基因型及等位基因频率在两组间比较差异有统计学意义(P<0.05);与rs2271289位点TT基因型相比,CC基因型是促进瘢痕妊娠发生的危险因素(OR=24.00,95%CI:1.74~330.80);与rs2271289位点T等位基因相比,C等位基因是促进瘢痕妊娠发生的危险因素(OR=3.12,95%CI:1.25~7.78)。rs2303579和rs10518830位点的基因型及等位基因频率在两组间比较差异无统计学意义(P>0.05)。结论NEDD4基因rs2271289位点的CC基因型及C等位基因可能是促进瘢痕妊娠发生的危险因素。

Objective To study the association between NEDD4 gene polymorphism and scar pregnancy.Methods Forty pregnant women who visited our hospital from January 2018 to September 2019 were selected as the research subjects.According to their ultrasound results,the patients were divided into the scar pregnancy group and the non-scar pregnancy group,with 20 cases in each group.Blood samples were drawn from cubital veins for extraction of DNA samples.The genetic loci of rs2271289,rs2303579 and rs10518830 of the NEDD4 gene were sequenced.The genotype and allele frequencies were compared between the two groups.Results The genotype and the allele frequency of rs2271289 locus were significantly different between the two groups(P<0.05).Compared with the TT genotype at rs2271289 locus,CC genotype was a risk factor for promoting scar pregnancy(OR=24.00,95%CI:1.74~330.80);compared with the T allele at rs2271289 locus,the C allele was a risk factor for promoting scar pregnancy(OR=3.12,95%CI:1.25~7.78).The genotypes and allele frequencies of rs2303579 locus and rs10518830 locus were not significantly different between the two groups(P>0.05).Conclusion The CC genotype and the C allele at rs2271289 locus of NEDD4 gene may be the risk factors for promoting scar pregnancy.