不同激素组合对蝴蝶兰快繁苗品质的影响

Effects on Quality Improvement of Phalaenopsis Fast-propagation Seedlings with Different Hormone Concentration Combinations

[目的]筛选不同激素组合对蝴蝶兰花梗侧芽诱导营养芽、幼叶诱导丛生芽、根尖诱导原球茎以及生根培养的最适宜培养基配方。[方法]通过诱导蝴蝶兰花梗上侧芽萌发,获得无菌蝴蝶兰营养芽,以营养芽上的茎尖诱导原球茎,从幼叶诱导丛生芽,建立蝴蝶兰无菌快繁培养体系,筛选出蝴蝶兰快繁各个环节的最佳培养基配方。[结果]筛选出适合蝴蝶兰花梗侧芽诱导营养芽、茎尖诱导原球茎状体、叶片诱导丛生芽、原球茎状体继代增殖、丛生芽继代增殖和幼苗生根培养最适宜培养基。[结论]为蝴蝶兰工厂化发展提供参考依据。

[Objective]The aim was to screen the optimum induction medium of pedicel lateral bud,multiple shoot,root tip,rooting culture in Phalaenopsis orchid.[Method]Using the stem tip of axenic adventitious bud to induce the protocorm and the young leaves of axenic adventitious bud to induce the cluster buds,which would establish axenic culture system,and screen out the ingredient of culture medium.[Result]Through the induction of sprout,the optimum medium were found for lateral bud of floral stalk,stem tip induction,axenic adventitious bud and young leaves of axenic adventitious bud.[Conclusion]The study could provide references for Phalaenopsis production development.