细胞因子和富血小板血浆对肌腱干细胞的影响

Effect of cytokines and platelet-rich plasma on tendon derived stem cells

背景:肌腱干细胞存在于肌腱中,对于肌腱修复具有独特的功能,不同细胞因子对肌腱干细胞的增殖、分化功能具有不同影响;富血小板血浆是指全血通过梯度离心分层后得到的富含血小板的血浆部分,含有多种较丰富的细胞因子,有助于促使韧带和肌腱再生。目的:探讨细胞因子和富血小板血浆对肌腱干细胞增殖、分化影响的最新进展。方法:由第一作者以"tendon derived stem cells,tendon stem/progenitor cells,tendon stem cell,platelet-rich plasma,ligament injury"为英文关键词,以"肌腱干细胞,富血小板血浆,肌腱损伤"为中文关键词。检索2007至2019年期间收录在PubMed、中国知网、万方等数据库中的文献,排除与文章研究目的无关及重复性的文献,纳入符合标准的83篇文献进行综述。结果与结论:肌腱干细胞是进行细胞移植治疗肌腱损伤的理想细胞,其增殖、分化受细胞因子影响,富血小板血浆包含大量细胞因子,具有刺激肌腱干细胞增殖、分化的功能,亦有成为细胞移植载体的潜力。探究细胞因子和富血小板血浆与肌腱干细胞增殖、分化的关系,将为肌腱干细胞的临床应用寻觅一条新途径。

BACKGROUND:Tendon derived stem cells exist in the tendon and have unique functions for tendon repair.Different cytokines have different effects on the proliferation and differentiation of tendon derived stem cells.Platelet-rich plasma refers to the blood product obtained from the whole blood through gradient centrifugation and stratification,which contains a variety of cytokines that could help to promote the regeneration of ligaments and tendons.OBJECTIVE:To investigate the latest progress of effects of cytokines and platelet-rich plasma on proliferation and differentiation of tendon derived stem cells.METHODS:Using“tendon derived stem cells,tendon stem/progenitor cells,tendon stem cell,platelet-rich plasma,ligament injury”as keywords in English and“tendon derived stem cells,platelet-rich plasma,ligament injury”in Chinese,the first author searched PubMed,CNKI,and Wanfang for relevant articles published from 2007 to 2019.Literature unrelated to the purpose of the study and repetitive literature were excluded,and 83 articles that meet the criteria were included for review.RESULTS AND CONCLUSION:Tendon derived stem cells are ideal cells for the treatment of tendon injury in cell transplantation.Its proliferation and differentiation are influenced by cytokines.Platelet-rich plasma contains a large number of cytokines,which can stimulate the proliferation and differentiation of tendon derived stem cells and have the potential to become a carrier of cell transplantation.Exploring the relationship between cytokines and proliferation and differentiation of tendon stem cells will provide a new approach for the clinical application of tendon derived stem cells.