姜黄素对大鼠子宫内膜异位症影响的实验研究

Experimental study on the effect of curcumin on endometriosis in rats

目的:分析姜黄素对大鼠子宫内膜异位组织转录因子Twist1、Twist2、E-钙黏蛋白(E-cadherin)、Syndecan-1表达的影响。方法:选取SPF级雌性SD大鼠采用自体内膜移植法建立子宫内膜异位症大鼠模型,将造模成功的30只大鼠随机分为模型组、低剂量姜黄素组(25 mg·kg^-1·d^-1)、中剂量姜黄素组(50 mg·kg^-1·d^-1)、高剂量姜黄素组(100 mg·kg^-1·d^-1)、孕三烯酮组(0.5 mg·kg^-1·d^-1),每组各6只,设立5只大鼠假手术组为空白对照组,记录各组大鼠异位内膜组织体积;采用免疫组化法检测各组大鼠子宫内膜异位组织Twist1、Twist2、E-cadherin、Syndecan-1表达的变化。结果:与模型组相比,姜黄素各剂量组和孕三烯酮组大鼠异位组织体积明显较低,差异具有统计学意义(P<0.05);Twist1阳性主要定位于细胞核中,Twist2阳性主要定位于细胞质中,E-cadherin、Syndecan-1阳性染色主要定位于上皮细胞膜上,少见于个别细胞质。与模型组相比,姜黄素各剂量组和孕三烯酮组Twist1、Twist2、Syndecan-1评分均明显降低,E-cadherin评分明显升高(P<0.05),低中剂量姜黄素组Twist1、Twist2、Syndecan-1评分明显高于孕三烯酮组,E-cadherin评分低于孕三烯酮组(P<0.05),高剂量姜黄素组Twist1、Twist2、E-cadherin、Syndecan-1评分与孕三烯酮组比较差异无统计学意义(P>0.05)。结论:姜黄素可降低子宫内膜异位组织中Twist1、Twist2、Syndecan-1的表达,升高E-cadherin的表达,抑制模型大鼠异位内膜的生长,以达到治疗的目的。

AIM:To analyze the effect of curcumin on the expression of transcription factors Twist1,Twist2,E-cadherin and Syndecan-1 in rats with endometriosis.METHODS:Thirty SD rats were randomly divided into five groups:model group,low dose curcumin group(25 mg·kg^-1·d^-1),medium dose curcumin group(50 mg·kg^-1·d^-1),high dose curcumin group(100 mg·kg^-1·d^-1)and gestrinone group(0.5 mg·kg^-1·d^-1).There were 6 rats in each group,and 5 rats in sham operation group were set up as blank control group.The expression of Twist1,Twist2,E-cadherin and Syndecan-1 was detected by immunohistochemistry.RESULTS:Compared with the model group,the ectopic tissue volume of curcumin group and gestrinone group was significantly lower,the difference was statistically significant(P<0.05).Positive Twist1 was mainly located in the nucleus,positive Twist2 was mainly located in the cytoplasm,positive E-cadherin and Syndecan-1 staining was mainly located in the epithelial cell membrane,rarely in individual cytoplasm.Compared with the model group,the scores of Twist1,Twist2 and Syndecan-1 in each dose group of curcumin and gestrinone group were significantly lower,and the scores of Twist1,Twist2 and Syndecan-1 in low and middle dose curcumin group were significantly higher than those in gestrinone group,and the scores of E-cadherin were lower than those in gestrinone group(P<0.05).The scores of Twist1,Twist2,E-cadherin and Syndecan-1 in high dose curcumin group were significantly higher.There was no significant difference between the high dose curcumin group and gestrinone group(P>0.05).CONCLUSION:Curcumin can reduce the expression of Twist1,Twist2 and Syndecan-1,increase the expression of E-cadherin,and inhibit the growth of endometrium in the model rats,so as to achieve the purpose of treatment.